How can I simultaneously recognize unknown patterns and variables from a given data set?

20 January 2021 6,385 3 View

How to use Cantera's equilibrium program to calculate adiabatic temperature of dust flame?

To calulate the adiabatic flame temperature using Cantera, we need to provide a .cti file that stores all thermodynamic information needed. We can simply convert a Chemkin mechanism file to .cti...

13 January 2021 7,072 2 View

Can result from nanostring be analyzed in the connectivity map?

I have several study results of natural compounds which were generated by the nonostring. Since there are only limited information of these compound, I would like to set up some analysis of gene...

22 March 2020 6,858 3 View

How to use Diana software for finite element analysis of reinforced concrete frame?

Diana is a finite element analysis software , How to use Diana software correctly to conduct RC framework modeling with masonry filled walls, The finite element analysis and hysteretic curve of...

24 February 2020 5,223 1 View

batch exporting data; R language; How to fix this error ?

Hi, colleagues I got this error message when I tried to export my data from Rstudio. I have searched for solution but the problem remains. Could you help? Thanks "type" is the data list,...

23 December 2019 4,769 3 View

Could I extract RANK/RANKL/OPG mRNA from peripheral blood samples?

I want to know that whether it is possible to extract RANK/RANKL/OPG mRNA from peripheral blood samples instead of tissue homogenates. Thanks a lot.

07 September 2019 7,431 3 View

How to calculate the score of SF-12V2?

SF-12v2 is the latest method to study health, but its calculation method is difficult to obtain. I wonder if you can provide the calculation method of SF-12v2. Thank you very much.

17 June 2019 6,108 2 View

Why does a peak as water apper in GC-MS spectrum at retention time 21.9 & 22.1 when organic solvent analysis with following condition?

Injector @ 300 celcius Oven 100 (3min) Rate 10 C/min Final 300 (2min)

06 February 2019 3,858 3 View

ANN does not process information.It only represent information. ?

Are there any research improvements related to attention, emotions, motivation, pleasure, consciousness and personality developed using ANN.

21 August 2018 8,983 4 View

Does anyone have reading recommendations about thin-film growth?

I am trying to know more theories and mechanisms about thin film growth? Does anyone list some reading recommendation about it and give your reason why these books are better? I found a book...

28 September 2017 10,008 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

No title

28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View