OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

Interpreting regression findings prior to inclusion of control variable?

I'm looking for advice on interpreting a statistical relationship - it's for a paper I am reviewing. (Variable names have been changed). It was found that: [IV anxiety + control variables y and...

06 January 2021 558 3 View

Cognitive interviews and survey - mixed methods?

I would be grateful for some advice about how to classify my research. I have conducted two surveys and used cognitive interviews. The first survey was descriptive (quantitative), then I...

14 December 2020 1,810 4 View

Can someone help me with an extraction protocol?

Hi all, Does anyone have a protocol for extracting DNA from agarose that is only 120 bp without a kit? The protocols I have tried are for much larger fragments and it is just not working. Thank you!

10 September 2020 1,291 2 View

Advice for getting dermatopathology tissue to stick to slides during immunostaining?

I work in a veterinary pathology lab and we do immunohistochemistry staining by hand on all types of tissues that are FFPE. We have particular difficulty getting dermatopathology (skin) samples to...

26 August 2020 792 1 View

Validation of experimental results?

Is it neccessary to perfrom validation of process model established using a box-benkhen experimental design? How "big mistake" will it be if I finish the experiment after performing the runs...

24 August 2020 6,917 3 View

Any reliable protocols for tissue detection of DNA damage with IHC or IF?

Dear all, Hope as a community your are doing fine. I have a question regarding antibodies against gH2AX, XRCC1 and p53bp1 that have worked for tissue detection for either IHC or IF, using...

08 June 2020 1,461 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View