Which fungal primers are best to use for RNA?

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Basil A. Abbas

18S

Elizabeth Dent

Hi Basil Abbas. Could you explain why you believe 18S is best? I am comparing fungal communities after different moisture and temperature treatments.

Sandeep RAJ R

The best option is to develop nested pcr model please refer this article Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts