Plasmids have the different form of DNA (Nicked/Relaxed, Linear, and Supercoiled).
Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...
27 June 2024 1,221 1 View
I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.
23 June 2024 3,446 1 View
Hi all, We performed Yeast-Cell-Glucose-Uptake assay to estimate the antidiabetic potential of the test compound. The Metformin treated yeast cells, resulted in taking up more glucse than normal...
02 April 2024 7,657 2 View
pursuing phd on geospatial data security.suggest good paper on robust invisible blind watermarking for copyright protection
12 March 2024 4,917 2 View
in a community area, there is an multi microgrid systems, why we call it as multi microgrid system but not treated as single microgrid system. and difference between single microgrid and multi...
03 March 2024 9,306 1 View
I am facing an issue of wire bonding, trying to ball bond Au wire onto a metal stack/semiconductor (Au/Ti/GaN). Thickness of Au = 200 nm, Thickness of Ti = 20 nm. I observe that the metal...
07 January 2024 4,813 1 View
I am studying precision for my assay/kit with the following parameters followed: Panel members to be tested: 1. Weak positive 2. Medium positive 3. Strong positive 4. Negative All these were...
11 December 2023 1,812 1 View
how can i add all my articles and journals in research gate including those published in research gate?
22 November 2023 5,926 3 View
Atorvastatin used to treat Atherosclerosis, seems to causes hepatoxicity upon prolonged usage. So, to inhibit such problem we have introduced an natural substances which is reported for the...
09 November 2023 587 0 View
I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?
09 November 2023 8,266 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View