Do you know any paper about the role of digital content making and translation marketing?

I am determined to write an article in this case and I wonder if anyone can help me in this case. Regards.

25 January 2021 3,330 3 View

How can I fix the floating node error in pspice?

I have a very large circuit and when I try to run a transient analysis of my circuit, there are so many floating node errors, this is the picture of a part of my circuit...

31 December 2020 6,053 5 View

Crop coefficiets (Kc) values more than 1 when estimating ETa using remote sensing?

Hello everyone, I was wondering if anyone knows how to deal with crops with Kc values of more than one when we want to estimate ETa using satellite images for different crops. IS there any...

10 December 2020 9,795 1 View

How Cryoprotectants works and prevent cell from damage due to freezing?

How these solvents limit shrinking ice crystals during freeze-drying or vitrification? and what are the main differences between DMSO and Glycerol? where each one is useful? I will be grateful if...

17 May 2020 3,540 1 View

The reason for the similarity of RMSF in the protein system and the protein-ligand complex?

The reason for the similarity of RMSF in the protein system and the protein-ligand complex

28 March 2020 3,784 3 View

Does the presence of clones on the agar plate mean that the cloning has been done successfully?

I did cloning. it was like a traditional approach. Firstly, Restriction digestion was performed for both vector (8kb) and target gen (700bp), and also the vectors and target gene were run on the...

21 March 2020 9,618 4 View

How can I start the simulation of esd effects on satellite solar panels? I'm looking for a start point for esd simulations by softwares likeCST,Hfss?

In all the papers the esd test model consists of a vaccum chamber in which samples exist. There is a plasma simulator or electron gun that simulates the space condition. these induce the esd on...

20 March 2020 5,938 3 View

What is the best method for measuring antifreeze compounds in insects?

I want to extract and study antifreeze compounds in diapause insects. What is the best method for it?

21 February 2020 3,321 2 View

How to solve linear regression equation with stochastic matrix using M-estimators with Huber objective function?

Suppose there is a regression model y=Ax+e which A is stochastic. and objective function is Huber function. as you see in attached file if A is deterministic equation (4-58) reduces to (4-60). but...

12 February 2020 6,518 2 View

Why is the DNA pellet dissolved hardly in the last step of miniprep?

29 January 2020 4,796 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 193 6 View