Which drug should I use as a positive control to have cytotoxic effect on CT26 cell line?

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How do you define three atoms for one box simulation?

I want to simulate two different ceramic structures. Each of the structures has a different number of atoms. I divided my simulation box into two parts. Now, in the create_atom section, I don't...

08 May 2024 9,647 2 View

What is correlation between anxiety and athlete’ performance in competitive sports?

Psychological factors significantly impact athletes' motivation and performance in competitive sports.

24 November 2023 1,015 2 View

Anyone who has experience in "GraphVar" Toolbox?

Hi there, I have some technical questions during the procedure of GraphVar for my EEG data.I would be happy if anyone can help me with "GraphVar". Thank you in advance for your help. Best, Neda

17 August 2023 821 0 View

SOS: A question from "GraphVar" Users?

Hi everyone, In the GUI, variable section, I created the variable sheet and I encounter an error while I execute it. I have attached screenshots of them. Any help would be really appreciated. Neda

16 August 2023 3,920 2 View

GraphVAr or Fieldtrip?

Hi everyone, I am working on EEG data and want to find functional connectivity and finally the graph. Would you please help me with which toolboxes are more useful to do this work? GraphVar or...

15 August 2023 6,837 2 View

How to mycoplasma culture in pplo broth ?

I want to enrich the semen and soap vaginal sample in mycoplasma culture medium with horse serum, arginine and phenol red. But I have a problem with how to cultivate. After consuming arginine in...

27 July 2023 2,439 0 View

What is the best housekeeping gene for QRT-PCR in breast cancer?

In research to investigate the expression of genes in breast cancer, which of the housekeeping genes is the best gene for QRT-PCR؟

21 July 2023 3,158 1 View

Do you have a suggestion for removing PVA from the foil?

Hello I electrospun PVA and then heat-treated it. Now, after the heat treatment, the fibers stick to the foil and do not separate. Do you have a suggestion for removing PVA from the foil?

05 July 2023 1,963 3 View

How can terminate Abaqus step when the failure criterion is reached?

the failure criterion is maximum stress in a specific cell

04 May 2023 3,347 1 View

What is the relation between approximate thickness and mid-surface offset parameter of TPMS unit cell and its relative density ?

The mathematical function of TPMS unit cell is as follows: (for example Gyroid) sin x * cos y+ sin y * cos z+ sin z * cos x = c parameter 𝑐 determines the relative density of the unit cell. I am...

29 March 2023 2,529 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

"Idrisi Selva" tutorial Books for pridiction with driving factors in modeling?

Some educational books with practical exercises of the "Idrisi Selva" software, which have step-by-step visual training for predicting the change of land use/land cover and including the use of...

28 July 2024 4,168 0 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View