30 September 2020 1 7K Report

I'm using Sarstedt plates and inserts for growing Caco-2 cells and I want to evaluate the monolayer integrity by measuring the TEER. For what I've learnt there are two TER-measurement devices: Millicell ERS-2 Voltohmmeter (Millipore) and EVOM2 epithelial voltmeter (WPI). Can anyone suggest me the right instrument and accessories (“chopstick” electrodes/ EndOhm chambers) compatible with Sarstedt inserts?

Secondly, I would like to know if it is possible to measure the TEER and perform paracellular permeability studies with Lucifer Yellow using the cell medium (DMEM).

Thank you!

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