I hope someone can help me with this! I am currently working with one enzyme expressed in E. coli. We want to scale up the process to an industrial production (no Academia involved).
The regulation of E. coli BL21 cells is not clear to me. Can be used for commercial/industrial use if used for enzyme production?
If not, what other bacteria can be used instead?
If we use a pET vector system, can this be used for industrial production as well?
Any discussion, advice, or document will be very much appreciated!
Thanks
Hi Irene,
E. coli BL21 is one of the most used strains for large-scale (industrial) production of recombinant proteins.
You can check the following link for other E. coli strains for the expression of heterologous proteins:
https://www.embl.org/groups/protein-expression-purification/services/protein-expression/e-coli-expression-strains/#:~:text=C41(DE3)%20and%20C43(,coli%20as%20well.
Or follow the paper below for more information:
Miroux B. and Walker J.E. (1996) Over-production of Proteins in Escherichia coli: Mutant Hosts that Allow Synthesis of some Membrane Proteins and Globular Proteins at High Levels J. Mol. Biol. 260: 289-298.
I have personal experience using different BL21, Rosetta2(DE3) and pLysS strains of E. coli, and all of them express heterologous proteins pretty well for different applications. E. coli Rosetta2(DE3) is another best strain that can be used for the expression of proteins having rare codons.
However, these strains have a major problem of endotoxins which are the unwanted by-products of recombinant proteins purified from any Escherichia coli. As endotoxin could interfere with in vitro biological assays and is the major pathological factor, it must be removed or inactivated before in vivo administration.
Hope the above link and the reference will be helpful for you.
Good Luck,
Faheem
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