In CD spectra, I am getting maxima at 185nm, 205nm, 222nm. Please suggest what could be the possible reason?

In my spectra of peptide-polymer, I am getting maxima at 185nm, 205nm and 222nm whereas peptide alone showed maxima at 202nm and 225nm. what could be these peaks corresponds to? Please suggest.

07 August 2019 7,641 3 View

How one can check the anti-microbial property of gel containing DMSO?

I am preparing gel in DMSO:Water (50:50 w/v). I want to check antimicrobial activity of this gel. Can anybody suggest how can i remove this DMSO from gel as i want to do antimicrobial assay in 96...

03 April 2016 6,151 2 View

Can anybody suggest me what precautions or care one should take for doing peptide (hydrophobic) RP-HPLC?

My peptide is 2000Da, hydrophobic and soluble only in DMF and DMSO. So, when I am doing RP-HPLC with Sunfire C-18 column (4.6x250mm, 5micron) with solvent system ACN+0.1%TFA and Water+0.1% TFA,...

01 February 2015 4,922 4 View

How can one select an appropriate HPLC column for peptide studies?

Can anybody help me in suggesting appropriate RP-HPLC column for hydrophobic peptide of molecular weight in range of 1.8-2.5KDa?

31 December 2014 2,854 2 View

Can anyone tell that what is the exclusion limit of sephadex LH 20 in organic solvents?

I want to purify hydrophobic peptide of molecular weight 2800 Da. Is sephadex LH appropriate for that? Moreover I want to know that what is the exclusion limit for Sephadex LH 20 in DMF as it is...

31 December 2014 5,249 3 View

Why would all prefer to use female athymic mice for invivo studies related to non-small cell lung cancer?

I have seen in most of the research articles that female nude (athymic) mice has been used mostly for invivo studies related to non small cell lung cancer. I have a question that why they used...

08 September 2014 2,921 6 View

Can we use GFP-Tag for peptide?

Green fluorescent protein is used to tagged proteins. Can i use it to tagged peptide? If yes, can I do it by conjugation or i need to take expression vector for it?

07 August 2014 1,507 5 View

How can I target a mitochondria in the cell? And what would be the pathway followed by a molecule targeted to the mitochondria?

Whether molecule is taken by lysosome only then targeted to mitochondria or directly taken up by mitochondria through different route? Please help.

07 August 2014 4,981 15 View

Does targeting to lysosome require targeting moiety and what would be the fate of any peptide taken up by a cell?

Is it true that any molecule like peptide is taken up by cell is through lysosome only. So if I need to target any molecule to lysosome and then want it to release into cytosol do it require the...

07 August 2014 7,381 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View