Kamal Malhotra

10 Questions 29 Answers 0 Followers

Questions related from Kamal Malhotra

Which counter ion (among acetate or formate) is recommended to replace TFA after peptide synethsis?

Hi, Can anyne suggest which is best counter ion (acetate/formate) to replace TFA after peptide synthesis when one has to work on cells and toxicity of TFA is a concern?

08 August 2019 4,154 8 View

In CD spectra, I am getting maxima at 185nm, 205nm, 222nm. Please suggest what could be the possible reason?

In my spectra of peptide-polymer, I am getting maxima at 185nm, 205nm and 222nm whereas peptide alone showed maxima at 202nm and 225nm. what could be these peaks corresponds to? Please suggest.

08 August 2019 7,710 3 View

How one can check the anti-microbial property of gel containing DMSO?

I am preparing gel in DMSO:Water (50:50 w/v). I want to check antimicrobial activity of this gel. Can anybody suggest how can i remove this DMSO from gel as i want to do antimicrobial assay in 96...

04 April 2016 6,210 2 View

Can anybody suggest me what precautions or care one should take for doing peptide (hydrophobic) RP-HPLC?

My peptide is 2000Da, hydrophobic and soluble only in DMF and DMSO. So, when I am doing RP-HPLC with Sunfire C-18 column (4.6x250mm, 5micron) with solvent system ACN+0.1%TFA and Water+0.1% TFA,...

02 February 2015 5,003 4 View

Can anyone tell that what is the exclusion limit of sephadex LH 20 in organic solvents?

I want to purify hydrophobic peptide of molecular weight 2800 Da. Is sephadex LH appropriate for that? Moreover I want to know that what is the exclusion limit for Sephadex LH 20 in DMF as it is...

01 January 2015 5,349 3 View

How can one select an appropriate HPLC column for peptide studies?

Can anybody help me in suggesting appropriate RP-HPLC column for hydrophobic peptide of molecular weight in range of 1.8-2.5KDa?

01 January 2015 2,916 2 View

Why would all prefer to use female athymic mice for invivo studies related to non-small cell lung cancer?

I have seen in most of the research articles that female nude (athymic) mice has been used mostly for invivo studies related to non small cell lung cancer. I have a question that why they used...

09 September 2014 2,979 6 View

Does targeting to lysosome require targeting moiety and what would be the fate of any peptide taken up by a cell?

Is it true that any molecule like peptide is taken up by cell is through lysosome only. So if I need to target any molecule to lysosome and then want it to release into cytosol do it require the...

08 August 2014 7,438 9 View

How can I target a mitochondria in the cell? And what would be the pathway followed by a molecule targeted to the mitochondria?

Whether molecule is taken by lysosome only then targeted to mitochondria or directly taken up by mitochondria through different route? Please help.

08 August 2014 5,037 15 View

Can we use GFP-Tag for peptide?

Green fluorescent protein is used to tagged proteins. Can i use it to tagged peptide? If yes, can I do it by conjugation or i need to take expression vector for it?

08 August 2014 1,576 5 View