How one can check the anti-microbial property of gel containing DMSO?

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In CD spectra, I am getting maxima at 185nm, 205nm, 222nm. Please suggest what could be the possible reason?

In my spectra of peptide-polymer, I am getting maxima at 185nm, 205nm and 222nm whereas peptide alone showed maxima at 202nm and 225nm. what could be these peaks corresponds to? Please suggest.

07 August 2019 7,641 3 View

Which counter ion (among acetate or formate) is recommended to replace TFA after peptide synethsis?

Hi, Can anyne suggest which is best counter ion (acetate/formate) to replace TFA after peptide synthesis when one has to work on cells and toxicity of TFA is a concern?

07 August 2019 4,025 8 View

Can anybody suggest me what precautions or care one should take for doing peptide (hydrophobic) RP-HPLC?

My peptide is 2000Da, hydrophobic and soluble only in DMF and DMSO. So, when I am doing RP-HPLC with Sunfire C-18 column (4.6x250mm, 5micron) with solvent system ACN+0.1%TFA and Water+0.1% TFA,...

01 February 2015 4,922 4 View

How can one select an appropriate HPLC column for peptide studies?

Can anybody help me in suggesting appropriate RP-HPLC column for hydrophobic peptide of molecular weight in range of 1.8-2.5KDa?

31 December 2014 2,854 2 View

Can anyone tell that what is the exclusion limit of sephadex LH 20 in organic solvents?

I want to purify hydrophobic peptide of molecular weight 2800 Da. Is sephadex LH appropriate for that? Moreover I want to know that what is the exclusion limit for Sephadex LH 20 in DMF as it is...

31 December 2014 5,249 3 View

Why would all prefer to use female athymic mice for invivo studies related to non-small cell lung cancer?

I have seen in most of the research articles that female nude (athymic) mice has been used mostly for invivo studies related to non small cell lung cancer. I have a question that why they used...

08 September 2014 2,921 6 View

Can we use GFP-Tag for peptide?

Green fluorescent protein is used to tagged proteins. Can i use it to tagged peptide? If yes, can I do it by conjugation or i need to take expression vector for it?

07 August 2014 1,507 5 View

How can I target a mitochondria in the cell? And what would be the pathway followed by a molecule targeted to the mitochondria?

Whether molecule is taken by lysosome only then targeted to mitochondria or directly taken up by mitochondria through different route? Please help.

07 August 2014 4,981 15 View

Does targeting to lysosome require targeting moiety and what would be the fate of any peptide taken up by a cell?

Is it true that any molecule like peptide is taken up by cell is through lysosome only. So if I need to target any molecule to lysosome and then want it to release into cytosol do it require the...

07 August 2014 7,381 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View