In CD spectra, I am getting maxima at 185nm, 205nm, 222nm. Please suggest what could be the possible reason?

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Which counter ion (among acetate or formate) is recommended to replace TFA after peptide synethsis?

Hi, Can anyne suggest which is best counter ion (acetate/formate) to replace TFA after peptide synthesis when one has to work on cells and toxicity of TFA is a concern?

07 August 2019 4,181 8 View

How one can check the anti-microbial property of gel containing DMSO?

I am preparing gel in DMSO:Water (50:50 w/v). I want to check antimicrobial activity of this gel. Can anybody suggest how can i remove this DMSO from gel as i want to do antimicrobial assay in 96...

03 April 2016 6,242 2 View

Can anybody suggest me what precautions or care one should take for doing peptide (hydrophobic) RP-HPLC?

My peptide is 2000Da, hydrophobic and soluble only in DMF and DMSO. So, when I am doing RP-HPLC with Sunfire C-18 column (4.6x250mm, 5micron) with solvent system ACN+0.1%TFA and Water+0.1% TFA,...

01 February 2015 5,021 4 View

How can one select an appropriate HPLC column for peptide studies?

Can anybody help me in suggesting appropriate RP-HPLC column for hydrophobic peptide of molecular weight in range of 1.8-2.5KDa?

31 December 2014 2,952 2 View

Can anyone tell that what is the exclusion limit of sephadex LH 20 in organic solvents?

I want to purify hydrophobic peptide of molecular weight 2800 Da. Is sephadex LH appropriate for that? Moreover I want to know that what is the exclusion limit for Sephadex LH 20 in DMF as it is...

31 December 2014 5,381 3 View

Why would all prefer to use female athymic mice for invivo studies related to non-small cell lung cancer?

I have seen in most of the research articles that female nude (athymic) mice has been used mostly for invivo studies related to non small cell lung cancer. I have a question that why they used...

08 September 2014 3,009 6 View

Can we use GFP-Tag for peptide?

Green fluorescent protein is used to tagged proteins. Can i use it to tagged peptide? If yes, can I do it by conjugation or i need to take expression vector for it?

07 August 2014 1,606 5 View

Does targeting to lysosome require targeting moiety and what would be the fate of any peptide taken up by a cell?

Is it true that any molecule like peptide is taken up by cell is through lysosome only. So if I need to target any molecule to lysosome and then want it to release into cytosol do it require the...

07 August 2014 7,466 9 View

How can I target a mitochondria in the cell? And what would be the pathway followed by a molecule targeted to the mitochondria?

Whether molecule is taken by lysosome only then targeted to mitochondria or directly taken up by mitochondria through different route? Please help.

07 August 2014 5,065 15 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

How to improve peaks separation in HPLC?

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30 July 2024 435 3 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

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16 July 2024 6,918 7 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What strains of C57 mice can induce rheumatoid arthritis?

Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...

09 July 2024 6,657 0 View

Bruce Neagle

These could be your solvents 'absorbance extinction coefficients'. As I recall 185 nm was Water and 205 nm was Methanol.

Julio Gonzales

I suggest you review the work of @Alvaro Sebastían Siano

Article Péptido Antimicrobiano Quimérico de Dermaseptina-S1 y Tigeri...

Kamal Malhotra

Hi Julio, Can you provide this article in English, please.

Thanks