Which counter ion (among acetate or formate) is recommended to replace TFA after peptide synethsis?

Hi, Can anyne suggest which is best counter ion (acetate/formate) to replace TFA after peptide synthesis when one has to work on cells and toxicity of TFA is a concern?

07 August 2019 4,025 8 View

How one can check the anti-microbial property of gel containing DMSO?

I am preparing gel in DMSO:Water (50:50 w/v). I want to check antimicrobial activity of this gel. Can anybody suggest how can i remove this DMSO from gel as i want to do antimicrobial assay in 96...

03 April 2016 6,151 2 View

Can anybody suggest me what precautions or care one should take for doing peptide (hydrophobic) RP-HPLC?

My peptide is 2000Da, hydrophobic and soluble only in DMF and DMSO. So, when I am doing RP-HPLC with Sunfire C-18 column (4.6x250mm, 5micron) with solvent system ACN+0.1%TFA and Water+0.1% TFA,...

01 February 2015 4,922 4 View

How can one select an appropriate HPLC column for peptide studies?

Can anybody help me in suggesting appropriate RP-HPLC column for hydrophobic peptide of molecular weight in range of 1.8-2.5KDa?

31 December 2014 2,854 2 View

Can anyone tell that what is the exclusion limit of sephadex LH 20 in organic solvents?

I want to purify hydrophobic peptide of molecular weight 2800 Da. Is sephadex LH appropriate for that? Moreover I want to know that what is the exclusion limit for Sephadex LH 20 in DMF as it is...

31 December 2014 5,249 3 View

Why would all prefer to use female athymic mice for invivo studies related to non-small cell lung cancer?

I have seen in most of the research articles that female nude (athymic) mice has been used mostly for invivo studies related to non small cell lung cancer. I have a question that why they used...

08 September 2014 2,921 6 View

Can we use GFP-Tag for peptide?

Green fluorescent protein is used to tagged proteins. Can i use it to tagged peptide? If yes, can I do it by conjugation or i need to take expression vector for it?

07 August 2014 1,507 5 View

How can I target a mitochondria in the cell? And what would be the pathway followed by a molecule targeted to the mitochondria?

Whether molecule is taken by lysosome only then targeted to mitochondria or directly taken up by mitochondria through different route? Please help.

07 August 2014 4,981 15 View

Does targeting to lysosome require targeting moiety and what would be the fate of any peptide taken up by a cell?

Is it true that any molecule like peptide is taken up by cell is through lysosome only. So if I need to target any molecule to lysosome and then want it to release into cytosol do it require the...

07 August 2014 7,381 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

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23 February 2021 4,014 3 View