Why is it necessary to incubate the FRAP Reagent at 37 degrees celcuis?

I prepared the FRAP reagent using a 10:1:1 ratio of 300 mM acetate buffer (pH 3.6), 10 mM TPTZ and 10 mM FeCl3.6H2O. Why is there a need to incubate the said solution at 37 degrees before adding...

01 February 2020 1,703 1 View

What is the typical solvent system for Diaion HP20?

Hello, I was wondering if you already have an experience in handling Diaion HP20, I would like to know if how big is the column size and the height that you use. And also if what solvent system do...

01 February 2016 5,989 0 View

Can I still isolate alkaloids if I partitioned an ethanolic extract in VLC using Silica gel HF254?

i partitioned an ethanolic extract of a plant that gave a positive indication for alkaloids in VLC using Silica gel HF254 with solvent systems Hex, DCM, Ethyl acetate and methanol.

06 July 2014 9,688 1 View

What is the typical solvent system for Diaion HP20?

Hi, i would like to ask if someone here has been able to use Diaion HP20 in their isolation. How is it packed (size of the column, height of the diaion hp20 in the column, and ID of the column)?...

06 July 2014 3,625 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View