Can I still isolate alkaloids if I partitioned an ethanolic extract in VLC using Silica gel HF254?

07 July 2014 1 10K Report

i partitioned an ethanolic extract of a plant that gave a positive indication for alkaloids in VLC using Silica gel HF254 with solvent systems Hex, DCM, Ethyl acetate and methanol.

Carla Wulandari Sabandar

You can check the present of alkaloids in each fraction by TLC and spray with specific reagents for alkaloid (ex. Dragendorf)

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