Why is it necessary to incubate the FRAP Reagent at 37 degrees celcuis?

I prepared the FRAP reagent using a 10:1:1 ratio of 300 mM acetate buffer (pH 3.6), 10 mM TPTZ and 10 mM FeCl3.6H2O. Why is there a need to incubate the said solution at 37 degrees before adding...

01 February 2020 1,703 1 View

Can I still isolate alkaloids if I partitioned an ethanolic extract in VLC using Silica gel HF254?

i partitioned an ethanolic extract of a plant that gave a positive indication for alkaloids in VLC using Silica gel HF254 with solvent systems Hex, DCM, Ethyl acetate and methanol.

06 July 2014 9,688 1 View

What is the typical solvent system for Diaion HP20?

Hi, i would like to ask if someone here has been able to use Diaion HP20 in their isolation. How is it packed (size of the column, height of the diaion hp20 in the column, and ID of the column)?...

06 July 2014 3,625 4 View

Which concentration of the test sample should be followed?

If I will conduct an assay in a microplate, which concentration of the test sample shall I follow? the indicated prepared concentration or the concentration together with the other additives (fluids)?

03 April 2013 786 6 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View