04 April 2013 1 4K Report

sRNA

More Zhi-nang Mao's questions See All
Avoid Sample Bias: How many subjects do we need for the underrepresented categories?

Hello, We have a 3*3*2*2*2*2*3*3= 3^4*2^4 (i.e. Total eight factors: four factors with two levels each, and four factors with three levels each) design. One of our factors is Age, which has three...

12 October 2020 6,389 2 View

How to use a mixed level fractional factorial design?

Hello, I have a 3*3*2*2*2*2*3*3= 3^4*2^4 (i.e. Total eight factors: four factors with two levels each, and four factors with three levels each). To get a small effect size in regression, the...

09 September 2020 1,946 10 View

Is the existing Quantum Computer able to break the current 2020 DLT/Blockchain encryption ?

According to Google, it can achieve a quantum dominance with 53 qubits, i.e. 253 calculation simultaneously. However, this is not sufficient to break SHA2562 encryption through brute force with...

31 July 2020 2,424 3 View

Sticky black, irregular patches in cell culture flask?

Hi all, Recently I encountered some irregular black/grey patches inside my cell culture flask (see attached image, x400 magnification). They are not motile, not floating, do not stick to cells...

08 July 2020 7,921 3 View

How to read .msp spectral library in R?

Hello everyone, Does anyone know how to read .msp files in R? I recently obtained a NIST library in MSP form by lib2nist. However, there is no retention indexes information in the MSP library. I...

25 February 2020 7,630 2 View

Shutdown of the BESA Research 6.0 when I apply the 'Automatic Artifact Correction' to correct artifacts of eye blinks in an 8-channel EEG data.?

Thank you for taking the time to read this. I have an EEG data recorded by an 8-channel wireless system. I want to correct the artifacts of eye blinks using the 'Automatic Artifact Correction'...

06 February 2020 4,744 1 View

Transfected cells died after trypsinization ?

Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...

21 December 2019 9,692 5 View

Problem with stable cell line generation?

Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...

19 September 2019 2,970 2 View

Does anyone familiar the restriction modification system in DH10B and DH5alpha E.coli strain?

I have plasmid DNA (BFP in pET vector) that minipreped from DH10B and DH5apha. There is no colony when I transformed DNA (from the DH10B) to MG1655, and many colonies when I transformed DNA (from...

17 September 2019 6,249 8 View

How does acridine orange works to recognize apoptosis, autophagy or lysosomal stability?

I used acridine orange staining to detect autophagy. First, the cells were grown in RPMI 10%FBS in confocal dishes, treated with compound with or without with wortmannin (0.5uM). Wortmannin was...

08 August 2019 9,319 5 View

Similar questions and discussions
Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

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