04 April 2013 1 4K Report

sRNA

More Zhi-nang Mao's questions See All
the silicon hydroxyl group of molecular sieve?

Can the acidity of the silicon hydroxyl group of zeolite, whether it is the terminal hydroxyl group or the silanol nest, catalyze the ethanol dehydrattion?

11 April 2024 1,235 0 View

Do you know the earliest activation time of microglia?

I am researching the microglia related neuroinflammation in CNS, and want to know the earliest time of microglia after acute inflammatory stimulation.

26 March 2024 6,209 1 View

Consequence of hyperuricaemia?

Other than common gout arthritis, I'd like to know more about the consequence of deposit MSU in organs.

14 July 2023 5,313 3 View

Wild type mouse T cell proliferation was induced by OVA?

Hello, I would like to ask you a question. Recently, I injected 100ug OVA subcutaneously into C57BL/6 mice and conducted enhanced immunization on the seventh day. On the 21st day, cells from the...

11 March 2023 7,801 0 View

How many alive single cells can be dissociated from one adult mouse hippocampus?

hi, I am trying to prepare single-cell suspension from an adult mouse hippocampus for single-cell sequencing, but now the total alive single-cell number is very low, so I was wondering in general...

01 February 2023 9,838 0 View

How many cells can be obtained from one adult mouse hippocampus tissue?

hi, I am trying to dissociate mouse hippocampus tissue into single cells with The Neural Tissue Dissociation Kits (130-092-628) and followed by cell debris removal, I was wondering how many alive...

31 January 2023 3,583 3 View

Why can't the fluorescence signal of CFSE-labeled cells (E, G-7-OVA) in peripheral blood, spleen and inguinal lymph of mice be detected?

Hello, everybody, I'd like to ask you a question. E.G. 7-OVA cells were labeled with 5uM CFSE at 37℃ for 10 minutes and injected into mice via caudal vein. Why was it that CFSE-labeled cell...

17 November 2022 8,766 2 View

Are there any organic solvents which can dissolve lithium carbonate?

I'm trying to figure out a way to dissolve lithium carbonate without water.

17 October 2022 7,227 1 View

How to mechanically exfoliate WTe2 (Tungsten Ditelluride) on SiO2?

I'm working on the exfoliation of monolayer WTe2 on SiO2 substrate in order to create a heterostructure for transport measurements, and I'm having trouble getting thin layers. I tried various...

04 September 2022 3,455 5 View

Immunofluorescence of 3D spheroid embedded in collagen I?

I am very interested in performing the immunofluorescence staining on a 3D spheroid model embedded in collagen type I. For example, Figure 2 A&D from the article Hypoxia Induces a HIF-1-Dependent...

28 July 2022 5,530 2 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

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