Which company do you recommend to sequence the exome of tumor samples?

More Victor Martinez-Glez's questions See All

How can I improve the extraction of the DNA fraction (using TRIzol) in OCT embedded samples?

I'm performing simultaneous extraction of DNA / RNA / PROT with Trizol, in samples embedded in OCT. The results for RNA and PROT are good, but I'm having problems with the yield and quality of the...

02 March 2013 1,355 10 View

NGS molecular diagnosis, will be less useful dysmorphology experts?

I think not, although the NGS allows the study of multiple genes at once without going through the expert filter of a clinical geneticist, they will now be responsible for establishing...

01 February 2013 7,114 2 View

Can anyone recommend results or procedures about shallow foundations and shell foundations modeling with ABAQUS?

I'm working on a project of investigation on shell foundations behavior for tower structures, as a particular case of shallow foundations, modelling with ABAQUS code. I need to compare my results,...

01 January 1970 8,795 4 View

How do I determine the bearing capacity of shallow foundations modeled in ABAQUS?

I am modeling shallow and shell foundations on sand using ABAQUS/CAE software. I'm modeling the soil as elastic - plastic Mohr Coulomb and linear - structures with frictional interface between the...

01 January 1970 4,174 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Diogo Pinho

Dear Victor,

Sequencing Advanced Services Unit of Biocant (Cantanhede, Portugal) is a company that is reliable and affordable and they have experience with transcriptome sequencing.

Please contact [email protected] for experimental design and costs of your application.

Best regards,

Inmaculada Ibáñez de Cáceres

You can also contact with Ana Dopazo at CNIC. If you need further information, just contact me.