Which company do you recommend to sequence the exome of tumor samples?

More Victor Martinez-Glez's questions See All

What values of the empirical threshold of heavy rainfall can we adopt, for slope failure analysis, considering partially saturated soils?

We are working on the analysis of slope failure due to heavy rains, considering the initial condition of partially saturated soils. We need to know criteria for limit accumulated rainfall,...

16 November 2020 7,863 11 View

How or where to obtain functions of volumetric water content and unsaturated permeability vs matric suction, of soils used in earth dams building?

I need some functions of volumetric water content vs matric suction, and unsaturated permeability vs matric suction, of representative soils used in earth dam construction. Will it be possible to...

20 August 2020 7,254 4 View

I am looking for a software that integrates all the data of the technical control that is carried out in the earth dams. Could someone help me?

I work in the updating and improvement of the technical controls to earth dams in Cuba, integrating the results that are measured regularly in this process. I want to integrate these results under...

18 October 2018 3,772 3 View

What is the best method for NGS studies in regions with pseudogenes?

If I want to study several genes by NGS but most of them have one or more large regions with high homology to other regions of the genome, which capture method should be used. Could a Long Range...

03 September 2015 2,077 3 View

How can I improve the extraction of the DNA fraction (using TRIzol) in OCT embedded samples?

I'm performing simultaneous extraction of DNA / RNA / PROT with Trizol, in samples embedded in OCT. The results for RNA and PROT are good, but I'm having problems with the yield and quality of the...

02 March 2013 1,462 10 View

NGS molecular diagnosis, will be less useful dysmorphology experts?

I think not, although the NGS allows the study of multiple genes at once without going through the expert filter of a clinical geneticist, they will now be responsible for establishing...

01 February 2013 7,239 2 View

Can anyone recommend results or procedures about shallow foundations and shell foundations modeling with ABAQUS?

I'm working on a project of investigation on shell foundations behavior for tower structures, as a particular case of shallow foundations, modelling with ABAQUS code. I need to compare my results,...

01 January 1970 8,929 4 View

How do I determine the bearing capacity of shallow foundations modeled in ABAQUS?

I am modeling shallow and shell foundations on sand using ABAQUS/CAE software. I'm modeling the soil as elastic - plastic Mohr Coulomb and linear - structures with frictional interface between the...

01 January 1970 4,321 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Diogo Pinho

Dear Victor,

Sequencing Advanced Services Unit of Biocant (Cantanhede, Portugal) is a company that is reliable and affordable and they have experience with transcriptome sequencing.

Please contact [email protected] for experimental design and costs of your application.

Best regards,

Victor Martinez-Glez

Obrigado Diogo. I'll contact soon.

Inmaculada Ibáñez de Cáceres

You can also contact with Ana Dopazo at CNIC. If you need further information, just contact me.