What is the best method for NGS studies in regions with pseudogenes?

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Does 3T3-L1 or H9C2 cell line can be used for atherosclerosis model study?

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Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

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¿ Algún experto en simulación de inversores ?

Simular una topología de inversor para aplicaciones de vehículos eléctricos en el software PSIM

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What do old testament sanctuary system mean?

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How to learn more about SPSS and its Application?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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Baseline drift in HPLC? What causes this?

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Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

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How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

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How are iso-frequency contours plotted?

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How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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Juan Luis Mateo

Hi Victor,

I think that Long Range Multiplex PCR can be used, but have you considered generating BAC clones as well?

Best,

Juan

Victor Martinez-Glez

Thanks Juan,

I will consider that possibility.

Thanks Mathew.

MinION sounds good, but PacBio, if not mistaken, would require equipment that I do not have so would be forced to outsource and that's not my intention. In any case, I will review the two options thoroughly.