What am I doing wrong in ORR electrochemistry?

Hello I've recently reached the point in my work when I will need to conduct some electrochemical measurements, but unfortunately I have to start everything from scratch, because in our lab no one...

01 December 2020 3,447 3 View

Can I modify beginning of pelB/ompA tag as described?

Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...

25 November 2020 8,386 3 View

Can I use an ELISA kit dedicated to plasma obtained with citrate or heparin if I used EDTA for this purpose?

Good morning all, My question is just like in title. I was using EDTA when collecting mouse blood, and most of ELISA kits are dedicated for plasma gained from blood collected with citrate or...

15 April 2019 2,737 2 View

Can I use my frozen samples to mark macrophages?

Hello all, my question is about using my frozen samples to detect macrophages. I have uterine cells, scratched with 40um strainer and frozen in -81 degrees in 90% FBS and 10% DMSO medium. They...

31 March 2019 1,837 3 View

What microscope setup do I need for comet assay?

10 September 2017 5,189 2 View

Could you give me a hand with my electrophoresis?

I've been performing RAPD-PCR for the yeast cultures I isolated from the Sauerkraut and everything was great. Subsequently I started to perform RAPD-PCR for the bacterial cultures I got from the...

08 August 2015 607 11 View

Is anyone using ELPHY Plus Ver. 5.0 to do the EBL?

27 January 2015 1,726 3 View

What are the best PCR-RAPD parameters to set when investigating Lactic Acid Bacteria?

I'm looking for best parameters for the PCR as well as best primers which I can use while investigating lactic acid bacteria. I was thinking of using M13 as a primer sequence, but I'd be glad if...

14 December 2014 6,658 3 View

Do you know any methods for learning from small data sets (and reasoning with such imperfect models)?

I am looking for solutions similar to leaky Noisy-OR gates in Bayesian networks, that allow for handling uncertainty caused by small amount of training data (see attached publication). The...

01 March 2014 3,282 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View