What am I doing wrong in ORR electrochemistry?

Hello I've recently reached the point in my work when I will need to conduct some electrochemical measurements, but unfortunately I have to start everything from scratch, because in our lab no one...

01 December 2020 3,447 3 View

Can I modify beginning of pelB/ompA tag as described?

Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...

25 November 2020 8,386 3 View

Can I use an ELISA kit dedicated to plasma obtained with citrate or heparin if I used EDTA for this purpose?

Good morning all, My question is just like in title. I was using EDTA when collecting mouse blood, and most of ELISA kits are dedicated for plasma gained from blood collected with citrate or...

15 April 2019 2,737 2 View

Can I use my frozen samples to mark macrophages?

Hello all, my question is about using my frozen samples to detect macrophages. I have uterine cells, scratched with 40um strainer and frozen in -81 degrees in 90% FBS and 10% DMSO medium. They...

31 March 2019 1,837 3 View

What microscope setup do I need for comet assay?

10 September 2017 5,189 2 View

Could you give me a hand with my electrophoresis?

I've been performing RAPD-PCR for the yeast cultures I isolated from the Sauerkraut and everything was great. Subsequently I started to perform RAPD-PCR for the bacterial cultures I got from the...

08 August 2015 607 11 View

Is anyone using ELPHY Plus Ver. 5.0 to do the EBL?

27 January 2015 1,726 3 View

What are the best PCR-RAPD parameters to set when investigating Lactic Acid Bacteria?

I'm looking for best parameters for the PCR as well as best primers which I can use while investigating lactic acid bacteria. I was thinking of using M13 as a primer sequence, but I'd be glad if...

14 December 2014 6,658 3 View

Do you know any methods for learning from small data sets (and reasoning with such imperfect models)?

I am looking for solutions similar to leaky Noisy-OR gates in Bayesian networks, that allow for handling uncertainty caused by small amount of training data (see attached publication). The...

01 March 2014 3,282 5 View

How can psychoanalysis make solution for social issue?

how can we use psychoanalytic perspective for describing, meaning, understanding and solving social problems(such as ethical problems, political problems, ...)?

27 December 2020 9,314 13 View

Having trouble finding the eigenvectors of a complex eigenvalues of a anisotropic flexible supports?

08 November 2020 1,819 2 View

Why are all my Eigenvalues from the Parallel Analysis using a Principal Axis Factoring lower than 1?

Hi, I am running an Exploratory Factor Analysis (EFA) on SPSS and trying different extraction methods. The Principal Component Analysis (PCA) suggested 4 factors whose Eigenvalue (EV>1). The...

11 October 2020 7,870 9 View

In simple terms: to what extent do you believe classicial inversion frameworks provide more "general" estimates than learned frameworks?

This is more of a survey question than a query for precise mathematical detailing. Opinions are welcome!

10 September 2020 6,516 2 View

For every positive even integer k, can we get a graph(not necessarily simple) whose first Zagreb index is k?

I'm working on the inverse problems on topological induces. Actually I got a partial answer for my question. But I have to generalize this result. Please help me in this regard. Thank you SMH

13 July 2020 5,010 2 View

Is there any graph whose adjacency spectral radius is maximum degree and least eigenvalue is minimum degree?

In my opinion we cannot find characterization of such graphs in the literature.

13 July 2020 6,509 6 View

How should I initialise the mpirun parameters for running a quantum espresso 'scf' calculation for the best parallel computing performance?

Hi, I am trying to do a Quantum espresso SCF calculation on an Intel Xeon Gold Gold 5120 CPU @ 2.20 GHz (2 Processor). It has 56 cores and a 96 GB RAM. I am trying to do a parallel calculation...

01 July 2020 6,821 3 View

What are the best (medical) image processing conferences?

Hi, I am interested in image reconstruction and I mostly go to IEEE NSS-MIC and Fully3D I would like to attend to new conferences about applied mathematics for image processing, with topics such...

09 March 2020 5,978 3 View

How to scale the time of a discrete-time state-space model in order to get a better conditioned model (how to do it for a continuous model is known)?

In order to get a better conditioned A matrix, the absolute mean of the eigenvalues of the A matrix should be one (all eigenvalues are between -1 and 1, so within the unit circle and the absolute...

21 February 2020 9,864 5 View

How to scale the time of a discrete-time state-space model in order to get a better conditioned model (how to do it for a continuous model is known)?

In order to get a better conditioned A matrix, the absolute mean of the eigenvalues of the A matrix should be one (all eigenvalues are between -1 and 1, so within the unit circle and the absolute...

01 February 2020 5,712 5 View