How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

Hi everyone, how can we remove the snow-covered areas in global scale?

We obtained the global results using remote sensing reanalysis dataset. But the snow-coverd areas should be removed (like the blank area in the picture below, sources: Purdy et al., 2016). Does...

20 September 2020 3,463 5 View

How to add a scale bar for images acquired from Molecular Device Imagexpress pico?

I saved some images when I scanned certain area using imagexpress pico. But don't know how to add scale bar either in system or image J. I am wondering if anybody can provide some help on that?

17 September 2020 8,068 4 View

How should I design the N-terminal VC plasmid of BiFC assay?

I'm going to detect protein-protein interaction with BiFC assays and my target proteins are ER retention with KDEL motif. so I want to fuse VC to the N-terminal of my protein. My question is if...

22 July 2020 3,456 1 View

How to grow more BL21 auxothrophic strain cells?

Dear all, I have just purchased a BL21 auxothrophic strain (RF12) from Addgene. The cells come in the form of agar stab. I intend to grow more cells which will be ready to used for protein...

11 May 2020 8,384 3 View

Roche has stopped its Universal Probe primer design service, does anyone know where or how we can design primers to fit Universal Probe Library?

Roche announced the Universal Probe primer design service will be stop in the end of 2020, but the primer design website has already been shutted...

10 February 2020 4,745 2 View

How do we make multi Fatsa files?

Hi, previously I had used MUMer to run but the input are only able to upload 1 Fasta file. When I test run a reference and the sample it work great but when I combine my sample 1 and sample 2 data...

25 July 2019 3,681 1 View

Does anyone know of a cancer cell line with c-myc gene amplified?

Breast cancer cells preferred, but other cell lines work too. Thank you.

06 May 2019 5,018 4 View

Is it possible and beneficial to learn probability distribution of input data when we apply machine learning methods like artificial neural network?

Hello, everyone! In recent time I am learning an online course called "learning from data". The lecturer showed the below learning diagram you can find from this website:...

29 October 2018 2,510 3 View

How to select the internal reference for the pseudo reference electrode?

Dear all, While use the pseudo reference electrode, internal reference need to introduce. In the non-aqueous electrochemical system, ferrocene is most used in literature. However, in aqueous...

30 July 2018 3,746 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View