What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Can anyone help me, i am trying to calculate mean linear intercept in my histological slides ?

in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...

11 December 2020 9,559 3 View

Can anyone help me with the protocol of intranasal administration to mice ?

i wanna administer cigarette smoke extract to induce emphysema in mice, i am asking for the maximum volume of intranasal administration ? can i use ether or chlorofom to anesthetize mice?

10 December 2020 6,319 4 View

Links of articles in Google Scholar?

In a scholar profile, in google scholar, when clicking on a link of an article for example, why some articles have a description and link to the page of the journal that publishes that article...

16 November 2020 5,216 4 View

Have you a word or LaTex Elsevier template?

Hello everyone, Hope you are very well. I just wonder if there is a word or LaTex Elsevier template for these two...

06 November 2020 4,504 3 View

How we can design hybrid system between deep learning and swarm intelligence system or deep learning with fuzz?

Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...

08 October 2020 4,943 3 View

Acidic hydrogel kills cells?

As hydrogel was prepared in acidic medium, released acid from hydrogel kills cells in in-vitro test. I am wondering to compromise such issue.

12 August 2020 9,319 3 View

Western blot : Empty well presenting signal ?

Dear all, I am currently performing some western blots in a big wells gel (40ul capacity) and i am filling 40ul in each well. My problem is that two of the last wells supposed to be empty...

10 August 2020 4,613 4 View

Mention the name of parasitic worm that causes river blindness?

mention the name of parasitic worm that causes river blindness?

04 July 2020 6,066 8 View

How can I create pre- and post-holiday dummy variables in EViews or Stata?

I have daily closing values of multiple stock indices and i have lists of public holidays for the whole period. I want to inspect the existence of a holiday effect. My regression equation is as...

23 May 2020 5,456 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View