Hi,

Probably it should be helpful source for you .....

https://www.researchgate.net/publication/20963749_Animal_models_for_intranasal_drug_delivery_studies_A_review_article

Such protocol was proposed in:

http://www.uab.edu/luckielab/protocols/ininnoc.doc

"

Protocol for intranasal inoculation of mice

2 ml Ketamine HCl (100mg/ml)

0.5 ml Xylazine (20mg/ml)

7.5 ml D.water

Sometimes, depending on the weight of the mouse, the amount of anaesthesia can vary from 75 to 150 ul.

3. After the mouse is deeply anaesthetized (confirmed by the absence of reflexes on the footpad), the mouse is held by it’s ears and 20 ul of the inoculum is gradually released into the nostrils (10 ul in each nostril) with the help of a micropipette. Adjust the rate of release so as to allow the mouse to inhale the inoculum without trying to form bubbles. This should cause a rapid increase in the breathing rate.

4. Hold the mouse in the hanging position for another couple of minutes till its breathing gradually returns to normal.

5. The control mice were inoculated intranasally with 20 ul of PBS."

Another protocol see below:

https://www.ltk.uzh.ch/dam/jcr:bc21156d-2cd8-404f-9bf1-a8b6227557a1/SOP-LTK-RES-74-B-EN%20intranasal%20application.pdf

and

http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf

Best regards

Tomasz

thank you sir Tomasz Grabowski

also these is videos are helpful:

https://www.jove.com/v/4440/intranasal-administration-of-cns-therapeutics-to-awake-mice

https://www.jove.com/t/59201/a-positioning-device-for-placement-mice-during-intranasal-sirna

thank you