Which CD44 variant can be used as intestinal stem cell marker for immunofluorescence?

More Samina Momtaz's questions See All

Protocol for slit lamp imaging for rabbit?

How can I set up a rabbit on a commercial slit lamp? Is there any specific restraining device/equipment involved? TIA

12 December 2023 7,557 0 View

How to solubilize GST tagged protein?

Hello, I was working to purify GST-tagged proteins expressed in BL21-DE3 strains. I was having some trouble with the formation of viscous stuff following sonication for the first couple of...

16 April 2023 7,584 3 View

What could be the reason for getting basal OCR too high (around 300-500 pmol/min) in Seahorse ATP rate assay?

I recently ran an ATP rate assay using the Seahorse XFp mini plate (8 wells). I got OCR values way higher than I typically see in publications. Basal OCR for control and experimental samples...

23 February 2023 8,256 3 View

Is there any technique to normalize Seahorse data without using BioTek Cytation 1 or 5 system?

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19 February 2023 8,991 6 View

Does anyone having problem with seeing only red puncta with PkhLC3 plasmid?

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15 June 2022 4,605 0 View

Does anyone having white precipitates after adding Pierce reagent?

Hello, I was using RIPA for protein lysis. I have recently seen a white precipitation formation after adding the Pierce reagent. We tried dilution of protein but still saw that white formation....

09 May 2022 9,170 6 View

Is there any common solvent of Carboxy methyl cellulose and PCL?

I am wondering if CMC and PCL can be electrospun together using a common solvent

30 October 2021 3,793 4 View

Can anyone tell me how can set the data in MS EXCEL or SPSS to run Rothamsted Carbon model?

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30 August 2021 6,050 1 View

Solubility of weed biochar for NMR analysis?

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26 April 2021 5,254 3 View

Dose any-one knows about cleaning validation for pathogens from fermentor?

Hi Dose any-one knows about cleaning validation protocols for pathogen-bacteria from fermentor? In industerial size, 1000 lit?

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What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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09 August 2024 9,621 0 View

Could it be a cell culture contamination?

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09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Monica Gabola

Hello!

I am using the cd44v6 variant, it is working quite well at least for the colon;

if you need more information please contact me;

cheers

Samina Momtaz

Hi Monica,

Thank you so much for your suggestion. We will try that. I will contact you if we need any further info. Thanks!

Samina.