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Questions related from Samina Momtaz
Hello, I was working to purify GST-tagged proteins expressed in BL21-DE3 strains. I was having some trouble with the formation of viscous stuff following sonication for the first couple of...
17 April 2023 7,569 3 View
I recently ran an ATP rate assay using the Seahorse XFp mini plate (8 wells). I got OCR values way higher than I typically see in publications. Basal OCR for control and experimental samples...
24 February 2023 8,250 3 View
Hello, We're going to perform a Seahorse assay using XFp 8-well system. The cell density will be 20,000 cells/well. We don't have the Cytation system to perform automated cell counts. As well as,...
20 February 2023 8,983 6 View
PKhLC3 plasmid is to observe the autophagic dynamics. The EGFP-Phluorin will disappear if the autophagosome fuses with the lysosome due to the acidic pH of the lysosome. So we expect to see only...
16 June 2022 4,598 0 View
Hello, I was using RIPA for protein lysis. I have recently seen a white precipitation formation after adding the Pierce reagent. We tried dilution of protein but still saw that white formation....
10 May 2022 9,162 6 View
We have IM7 that can recognize a common epitope for all the variants. It's no good for us for immunofluorescence. We will appreciate any advice or suggestions regarding this. Thank you!
04 May 2021 7,142 2 View
