Solubility of weed biochar for NMR analysis?

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Protocol for slit lamp imaging for rabbit?

How can I set up a rabbit on a commercial slit lamp? Is there any specific restraining device/equipment involved? TIA

12 December 2023 7,557 0 View

How to solubilize GST tagged protein?

Hello, I was working to purify GST-tagged proteins expressed in BL21-DE3 strains. I was having some trouble with the formation of viscous stuff following sonication for the first couple of...

16 April 2023 7,584 3 View

What could be the reason for getting basal OCR too high (around 300-500 pmol/min) in Seahorse ATP rate assay?

I recently ran an ATP rate assay using the Seahorse XFp mini plate (8 wells). I got OCR values way higher than I typically see in publications. Basal OCR for control and experimental samples...

23 February 2023 8,256 3 View

Is there any technique to normalize Seahorse data without using BioTek Cytation 1 or 5 system?

Hello, We're going to perform a Seahorse assay using XFp 8-well system. The cell density will be 20,000 cells/well. We don't have the Cytation system to perform automated cell counts. As well as,...

19 February 2023 8,991 6 View

Does anyone having problem with seeing only red puncta with PkhLC3 plasmid?

PKhLC3 plasmid is to observe the autophagic dynamics. The EGFP-Phluorin will disappear if the autophagosome fuses with the lysosome due to the acidic pH of the lysosome. So we expect to see only...

15 June 2022 4,605 0 View

Does anyone having white precipitates after adding Pierce reagent?

Hello, I was using RIPA for protein lysis. I have recently seen a white precipitation formation after adding the Pierce reagent. We tried dilution of protein but still saw that white formation....

09 May 2022 9,170 6 View

Is there any common solvent of Carboxy methyl cellulose and PCL?

I am wondering if CMC and PCL can be electrospun together using a common solvent

30 October 2021 3,793 4 View

Can anyone tell me how can set the data in MS EXCEL or SPSS to run Rothamsted Carbon model?

I want to run rothasmated carbon model equation for data, So how will set the data in excel for the RothC model or SPSS?

30 August 2021 6,050 1 View

Which CD44 variant can be used as intestinal stem cell marker for immunofluorescence?

We have IM7 that can recognize a common epitope for all the variants. It's no good for us for immunofluorescence. We will appreciate any advice or suggestions regarding this. Thank you!

03 May 2021 7,149 2 View

How can I determine the enzyme concentration in a liquid??

Dear All I am working on the retention (Adsorbtion) of different enzymes on the surface of different materials including soil, zeolite and etc. I need some information about the determination of...

30 September 2016 3,235 2 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

What is the degassing temperature and time for biochar for BET surface area analysis if TGA is not possible?

Since BET and TGA both are very costly instruments and many places the facilities are not available (for large number of samples) hence a threshold degassing temperature and time is preferable

16 July 2024 8,112 2 View

How can magnetic Biochar remove dye pollutants, is this technology beneficial?

The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?

07 July 2024 8,118 1 View

Can biochar reduce carbon dioxide levels and role of biochar in the sustainability of agriculture and environment?

01 July 2024 4,757 7 View

What are the applications of biochar in environmental remediation and role of biochar in mitigation of climate change through carbon sequestration?

01 July 2024 9,300 5 View

What are the application of biochar in environmental remediation and role of biochar in mitigation of climate change through carbon sequestration?

26 June 2024 6,743 2 View

Does biochar increase soil carbon & role of biochar on GHGs emissions & carbon sequestration in soil opportunities for mitigating climate change?

Does biochar increase soil carbon and role of biochar on greenhouse gas emissions and carbon sequestration in soil opportunities for mitigating climate change?

26 June 2024 8,986 3 View