I want to check hydrogel swelling at different ph values..Can I use PBS buffer for ph 3-10 range by adjusting 1M HCl and 1M NaOH
Hello Diksha, try to use buffers with appropriate pH ranges instead of trying to adapt one buffer to all. The formation products that result from the acid- base reactions will influence your results especially when they were not part of the medium, even if they were, at undesired concentration. Recognize the importance of the pKa . Select a buffer that has a pKa value close to the middle of the range required. If you expect the pH to drop during the experiment, choose a buffer with a pKa slightly lower than the working pH. This will permit the buffering action to become more resistant to changes in hydrogen ion concentration as hydrogen ions are liberated. Conversely, if you expect the pH to rise during the experiment, choose a buffer with a pKa slightly higher than the working pH. For best results, the pKa of the buffer should not be affected significantly by buffer concentration, temperature, and the ionic constitution of the medium. Try to use buffers without mineral cations when appropriate.
A buffer is exactly that - it buffers the pH (please don’t write ‘ph’ - it displays a level of ignorance).
Buy in a pH 4, 7, and 9 buffer and use these.
Hello Diksha, try to use buffers with appropriate pH ranges instead of trying to adapt one buffer to all. The formation products that result from the acid- base reactions will influence your results especially when they were not part of the medium, even if they were, at undesired concentration. Recognize the importance of the pKa . Select a buffer that has a pKa value close to the middle of the range required. If you expect the pH to drop during the experiment, choose a buffer with a pKa slightly lower than the working pH. This will permit the buffering action to become more resistant to changes in hydrogen ion concentration as hydrogen ions are liberated. Conversely, if you expect the pH to rise during the experiment, choose a buffer with a pKa slightly higher than the working pH. For best results, the pKa of the buffer should not be affected significantly by buffer concentration, temperature, and the ionic constitution of the medium. Try to use buffers without mineral cations when appropriate.
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