Dear All,
- i have isolated protein from plants material (leaves or seeds) by Tris-Hcl buffer and precipitate it by 10% TCA, followed by centrifugation to get the pellet.
- i washed the pellet with solvent.
- i want to detect the protein/ Polypeptides by TLC and HPLC.
- please suggest me which buffer OR solvent i can use to dissolve this isolated protein which will not interfere with HPLC and TLC
Thank you.
Hi, for TLC methods, I think you can search the method(s) viaTLC Camag Data base, Best regards
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm
Hi , You can dissolve in 0.5M NaOH , It is soluble. I am not sure you will get intact protein
Dear Sir
Beside the intersting answers provided to you.Please, find in attach a Chapter on: Peptide and Amino Acids Separation and Identification from Natural Products Ion Neda, Paulina Vlazan, Raluca Oana Pop,Paula Sfarloaga, Ioan Grozescu and Adina-Elena Segneanu.
Good luck
Dear All,
thank you for your answers.
but in case of salts it is not recommended because it will inter with the bio activities of the proteins or polypeptides.
i hope that some one can suggest solvent other than salts.
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