Mohammed Alsaiqali

5 Questions 18 Answers 0 Followers

Questions related from Mohammed Alsaiqali

Which of the following buffers are suitable for elution of protein band from SDS - PAGE gel which can be used for biological activity & MS analysis?

Dear All, I would like to elute protein band from SDS-PAGE gel stained by coomassie brilliant blue r-250 stain. which will be used for biological activity & mass determination. So which of the...

06 June 2016 1,413 6 View

Which buffer or solvent can be used to dissolve protein isolated from plants by tris-Hcl buffer and precipitated by TCA 10% so I can use it for TLC?

Dear All, i have isolated protein from plants material (leaves or seeds) by Tris-Hcl buffer and precipitate it by 10% TCA, followed by centrifugation to get the pellet. i washed the pellet with...

06 June 2016 8,403 4 View

How we can maintain the biological activity of peptides after eluting it from the HPLC RP C18 column?

Dear All, I just have read the following statement, that to maintain the biological activity of peptides after RP HPLC elution. "after eluting fractions, the protein(s) or peptides of...

03 March 2016 5,057 5 View

Which buffer can be used to prepare stock solution of a lyophilized peptides extracted from plants for performing MIC?

please suggest me I extracted a peptides from herbal plants and lyophilized them. For minimal inhibitory concentration (MIC) determination, which buffer and at what pH can be used to prepare the...

02 February 2016 6,204 5 View

How can I lyophilize a plant sample containing ethanol?

Lyophilization of plant extracts.

04 April 2014 8,928 1 View