due to determining the aflatoxin content by TLC method I need a bacterial nutrient media which doesn't show any fluorescent properties?
I have tried Nutrient and LB but seemingly they have fluorescent properties.
can anyone please help me with that?
sir i think you can try with any msm. This may be helpful for you
To be correct: Aflatoxins are produced by fungi (Aspergillus spp) and not by bacteria.
Hi Ahad,
as Kees pointed out aflatoxins come from Aspergillus fungi not bacteria. In that case you could try Saboraud or Czapek-dox media. Otherwise a mineral salt medium (Bushnell-Haas?) with glucose or citrate would probably do it!
hope this helps,
cheers,
Richard
Dear Dr. Kan
Thank you for your answer but I forgot to mention that my project is studying the degradation of Aflatoxins by bacteria.
Regards
Hi Again,
bushnell-haas with a suitable carbon source (glucose?) depending on your bacterial species will not give any background fluorescence.
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