Simulated Body Fluid (SBF) is a solution that mimics the ionic composition of human blood plasma, and it is commonly used for bioactivity tests to evaluate the performance of biomaterials, such as implants or scaffolds.
To synthesize SBF, the following steps can be taken:
Prepare a stock solution of 1 M Tris(hydroxymethyl)aminomethane (Tris) at a pH of 7.4.
Add the following salts to the Tris stock solution, in the order listed, while stirring until each salt is fully dissolved:0.137 M sodium chloride (NaCl) 0.027 M potassium chloride (KCl) 0.014 M calcium chloride dihydrate (CaCl2 · 2H2O) 0.003 M magnesium chloride hexahydrate (MgCl2 · 6H2O) 0.05 M sodium hydrogen carbonate (NaHCO3) 0.05 M potassium dihydrogen phosphate (KH2PO4)
Adjust the pH of the solution to 7.4 using hydrochloric acid (HCl) or sodium hydroxide (NaOH).
If your synthesized solution has a high pH of 8 or above, it suggests that the pH adjustment step was not done correctly. To fix this problem, you can add a small amount of HCl to lower the pH to the desired range of 7.2-7.4. You can then use a pH meter to verify the pH and make additional adjustments as needed. It is important to note that the pH of SBF is critical for its bioactivity and should be carefully monitored and adjusted.