Hello!.

I have to express a protein in S. cerevisiae with pYES2 vector.

This protein has a molecular weight of 56 kDa and a His tag for the purpose of purification with ProBond resin.

In the process of purifying the protein, the purified protein has a molecular weight of 30 kDa.

Which are the possible sources of error in my poor expression protein of interest?

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