Diego Andres Díaz-Dinamarca

4 Questions 7 Answers 0 Followers

Questions related from Diego Andres Díaz-Dinamarca

Is the "Violet Proliferation Dye 450, BD" compatible for T-cell adoptive transfer assays?

The carboxyfluorescein diacetate succinimidyl ester (CFSE) it is widely used to monitor the proliferation history of adoptively transferred T cells. In my case, I have the Violet Proliferation Dye...

11 November 2017 7,984 1 View

Could anyone let me know efficient method of doing colony qPCR in Streptococcus agalactiae?

I am following this protocol. Cells from a colony is picked up from agar plates by using a sterile pipette tip and resuspended into 20 μl of of distilled water. Vortex for 10 s for lysing cells....

02 February 2016 1,734 1 View

Which are the sources of error in the expression of proteins in yeast?

Hello!. I have to express a protein in S. cerevisiae with pYES2 vector.This protein has a molecular weight of 56 kDa and a His tag for the purpose of purification with ProBond resin.In the process...

01 January 2016 2,620 5 View

Does anyone know if the reagent Y-PER Yeast Protein Extraction Reagent is compatible with the purification column ProBond ™ Nickel-Chelating Resin?

Good day group! I have to purify a recombinant protein coupled to his-tag, wich is expressed in yeast. In my laboratory it is being evaluated as a method of analysis the "Y-PER Yeast Proteins...

12 December 2015 4,874 2 View