Could anyone let me know efficient method of doing colony qPCR in Streptococcus agalactiae?

01 February 2016 1,661 1 View

Which are the sources of error in the expression of proteins in yeast?

Hello!. I have to express a protein in S. cerevisiae with pYES2 vector.This protein has a molecular weight of 56 kDa and a His tag for the purpose of purification with ProBond resin.In the process...

24 January 2016 1,070 4 View

Which are the sources of error in the expression of proteins in yeast?

Hello!. I have to express a protein in S. cerevisiae with pYES2 vector.This protein has a molecular weight of 56 kDa and a His tag for the purpose of purification with ProBond resin.In the process...

31 December 2015 2,531 5 View

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Good day group! I have to purify a recombinant protein coupled to his-tag, wich is expressed in yeast. In my laboratory it is being evaluated as a method of analysis the "Y-PER Yeast Proteins...

11 December 2015 4,790 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View