11 November 2017 1 8K Report

The carboxyfluorescein diacetate succinimidyl ester (CFSE) it is widely used to monitor the proliferation history of adoptively transferred T cells. In my case, I have the Violet Proliferation Dye 450, BD. Can I use this dye instead of the CFSE?

More Diego Andres Díaz-Dinamarca's questions See All
Could anyone let me know efficient method of doing colony qPCR in Streptococcus agalactiae?

I am following this protocol. Cells from a colony is picked up from agar plates by using a sterile pipette tip and resuspended into 20 μl of of distilled water. Vortex for 10 s for lysing cells....

01 February 2016 1,762 1 View

Which are the sources of error in the expression of proteins in yeast?

Hello!. I have to express a protein in S. cerevisiae with pYES2 vector.This protein has a molecular weight of 56 kDa and a His tag for the purpose of purification with ProBond resin.In the process...

24 January 2016 1,258 4 View

Which are the sources of error in the expression of proteins in yeast?

Hello!. I have to express a protein in S. cerevisiae with pYES2 vector.This protein has a molecular weight of 56 kDa and a His tag for the purpose of purification with ProBond resin.In the process...

31 December 2015 2,670 5 View

Does anyone know if the reagent Y-PER Yeast Protein Extraction Reagent is compatible with the purification column ProBond ™ Nickel-Chelating Resin?

Good day group! I have to purify a recombinant protein coupled to his-tag, wich is expressed in yeast. In my laboratory it is being evaluated as a method of analysis the "Y-PER Yeast Proteins...

11 December 2015 4,908 2 View

Does anyone know if the reagent Y-PER Yeast Protein Extraction Reagent is compatible with the purification column ProBond ™ Nickel-Chelating Resin?

Good day group! I have to purify a recombinant protein coupled to his-tag, wich is expressed in yeast. In my laboratory it is being evaluated as a method of analysis the "Y-PER Yeast Proteins...

02 December 2015 9,824 2 View

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