Sneha Gupta

5 Questions 29 Answers 0 Followers

Questions related from Sneha Gupta

Gel fiiteration chromatography

Hey Can I use GE Superdex 200 Increase 10/300 GL coloumn on Äkta purifier with P960 pump?

05 May 2017 8,778 5 View

Anyone has worked wizth Äkta Purifier P960? I have a quick question

anyone has worked wizth Äkta Purifier P960? I have a quick question

05 May 2017 6,718 5 View

How to isolate protein of interest (untagged) after transient tranfection in mammalian cell line?

I am trying my luck in HEK293T cells, by transfection. I can express and detect Protein post transfection. But since my construct is untagged what methods can be employed tro prify it (keeping in...

07 July 2016 7,781 6 View

What are the major questions asked in MCh neurosurgery examinations for India?

I can not find any previous year paper would be a great help if I can know that what kind of questions are asked at KGMC/IMS BHU for Mch neurosurgery exam

06 June 2016 4,420 0 View

Problems with cell-free reaction setups.the positive control eGFP not working somehow. any hits?

the only pointers here are: 1. I use DTT free setup (because my constructs need no DTT) also I have glutathione in my master mix. will that affect the robust eGFP? what can be the possible...

06 June 2016 668 5 View