I am looking for chemicals that can be used in mammalian cell culture to enhance oxidative metabolism (TCA cycle).
Can NAD+ precursors (NR, for example) be used?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351101/
I think Species include oxygen radicals and reactive nonradicals like H2O2
Article Chemically-induced oxidative stress increases the vulnerabil...
Article NAD+ Metabolism and Oxidative Stress: The Golden Nucleotide ...
I used the Gibson assembly to join together two PCR fragments (4400 bp +770 bp). After transformation of E. coli with the Gibson mix, I isolated DNA from single colonies and I performed a...
28 March 2020 1,299 3 View
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I did several minipreps (different plasmids extracted from different cell lines) and when I run them on agarose gel 0.8% they don't look good (smear at high MW and it looks always like that). The...
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Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
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I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
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Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
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As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View