Whether Linear dsDNA amplification with Phi29 requires specific protocol?

07 January 2017 1 6K Report

We are using Lucigen Phi29 for specifically enriching circular ssDNA viral genomes in our samples, primed by Exo resistant random hexamers. The reaction is working fine. However, I wish to know if the same protocol can also cpy the linear dsDNA of the host? Or is there any other protocol for specific enrichment of viral linear dsDNA (5-10 Kb)? Any help with protocol or literature will be highly appreciated.

Sibnarayan Datta

Thank you Dr Mahmoudpour for your help. We will try with our samples.

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