What should be the appropriate composition of dinitro-salicylic acid reagent for reducing sugar determination?

10 November 2015 8,905 9 View

What is the impact of music on growth of microorganisms? Is there any relationship?

07 August 2015 3,772 13 View

How can we confirm that our enzyme is purified or partially purified?

Is that from single band of protein by SDS-PAGE where we confirm or any other method? Thank you in advance.

02 March 2015 8,218 8 View

Why those kinds of work are encouraged and rewarded?? There are many researches going on but a few of them are beneficial for mankind. Why?

We generally experienced this many time while attending a "seminars or conferences" that there are a few topics given by an eminent person or by a student that can be used for the mankind and many...

01 February 2015 1,290 0 View

What is the easiest and safe way to mutate any bacteria or fungus in lab condition?

10 November 2014 8,832 2 View

Is there any lab technique to prove our lipase enzyme can be used as detergent or a detergent additives?

Lipase we studied was cold and organic solvent tolerant. My question is what are the other tests can be done to prove that the above enzyme can be used in detergent industries for detergent...

10 November 2014 2,295 3 View

What is the major differences between powder and liquid detergents available in market?

It was saying by many that powder detergents are very toxic to delicate clothes and liquid detergents are soft detergents and good for cloth washing. Is this statement right ?? If yes what is the...

09 October 2014 5,084 1 View

Why is there a reduction in the production of lipase when there is an increase in oil concentration?

While optimizing the lipase production, oil concentrations (tributyrin oil) from 5-25% v/v were taken, and it was observed that enzyme activity was decreased with an increase in oil...

09 October 2014 3,672 7 View

What is the relationship between enzyme hydrolytic zone and growth of filamentous fungi in relation to temperature?

While working on filamentous fungi for the production of cellulase enzyme, more hydrolytic zone was seen in case of CMC plate incubating at 15 degree cel. than 35 degree cel. But the growth of...

08 September 2014 2,894 6 View

Cold and organic solvents tolerant lipase can be used in which industries?

In which industries or any applications? Please suggest if any?? I am working on lipase, after characterization it was more active at low temperature and can also tolerate many organic solvents...

08 September 2014 8,281 0 View

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02 March 2021 3,060 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View