What should be the appropriate composition of dinitro-salicylic acid reagent for reducing sugar determination?

10 November 2015 8,905 9 View

What is the impact of music on growth of microorganisms? Is there any relationship?

07 August 2015 3,772 13 View

How can we confirm that our enzyme is purified or partially purified?

Is that from single band of protein by SDS-PAGE where we confirm or any other method? Thank you in advance.

02 March 2015 8,218 8 View

Why those kinds of work are encouraged and rewarded?? There are many researches going on but a few of them are beneficial for mankind. Why?

We generally experienced this many time while attending a "seminars or conferences" that there are a few topics given by an eminent person or by a student that can be used for the mankind and many...

01 February 2015 1,290 0 View

What is the easiest and safe way to mutate any bacteria or fungus in lab condition?

10 November 2014 8,832 2 View

Is there any lab technique to prove our lipase enzyme can be used as detergent or a detergent additives?

Lipase we studied was cold and organic solvent tolerant. My question is what are the other tests can be done to prove that the above enzyme can be used in detergent industries for detergent...

10 November 2014 2,295 3 View

Where to submit industrially important organisms? What is the procedure of culture submission?

My isolated micro. are good producers of cold active enzymes like (lipase and endoglucanase). Where to submit those cultures? I heard about MTCC. Is there any other options for this? Which is the...

10 November 2014 9,860 2 View

Why is there a reduction in the production of lipase when there is an increase in oil concentration?

While optimizing the lipase production, oil concentrations (tributyrin oil) from 5-25% v/v were taken, and it was observed that enzyme activity was decreased with an increase in oil...

09 October 2014 3,672 7 View

What is the relationship between enzyme hydrolytic zone and growth of filamentous fungi in relation to temperature?

While working on filamentous fungi for the production of cellulase enzyme, more hydrolytic zone was seen in case of CMC plate incubating at 15 degree cel. than 35 degree cel. But the growth of...

08 September 2014 2,894 6 View

Cold and organic solvents tolerant lipase can be used in which industries?

In which industries or any applications? Please suggest if any?? I am working on lipase, after characterization it was more active at low temperature and can also tolerate many organic solvents...

08 September 2014 8,281 0 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View