I'm planning on substituting my media with a balanced mix of nucleosides. Any advice on concentrations or supplier?
Guobing Xiang
many companis have it. Do an internet search: Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100mM. 100 mM dNTP mix is suitable for use in polymerase chain reaction(PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, andTdT-tailing reactions.
dNTP Set (100 mM)
www.thermofisher.com/order/catalog/product/10297018
- dNTP Set (100 mM) https://www.thermofisher.com/order/catalog/product/10297018Description Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) inpurified water. Each nucleotide is at a concentration of 100 mM. 100 mM dNTP mix is suitable for use inpolymerase chain reaction (PCR), sequencing, fill-in reactions, nick …
- 100 mM dNTP Mix, 250ul of 100mM of Each dATP, dTTP, dGTP, … https://elabsaver.com/product/100-mm-dntp-mix100mM dNTP (250ul of 100mM of each dATP dTTP dGTP dCTP) in 4 tubes, 250 ul/tube (Cat# MB-100) Description: 100 mM dNTP set aqueous solutions (250ul) at pH 7.0 of each of dATP, dCTP, dGTP anddTTP, each in a separate vial.
- dNTP Set and dNTP Mix, PCR Grade - QIAGEN Online Shop https://www.qiagen.com/.../lab-essentials/buffers-reagents/dntp-set-and-dntp-mix-pcr-gradeEach individual 100 mM dNTP is supplied in water and can be diluted and mixed with any other dNTP tothe desired concentration. dNTP Mix, PCR Grade contains premixed dATP, dCTP, dGTP, and dTTP inwater (pH 7.5), each at a concentration of 10 mM.
- [PDF]100 mM dNTP Set, PCR Grade - Thermo Fisher Scientifictools.thermofisher.com/content/sfs/manuals/dntpset100mm_man.pdf100 mM dNTP (2'-deoxynucleoside 5'-triphosphate) Set consists of all four deoxynucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM.
0 votes
0 thanks
Nina Kozlova
Guobing Xiang, nucleoSides. Not dNTPs. I've solved the issue, thank you for your reply.
0 votes
0 thanks
Guobing Xiang
haha, sorry! I donot know people use nucleoside mix, I will check to see/to learn. Thank you!
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- DNA
More Nina Kozlova's questions See All
Similar questions and discussions