Is there a way to sequence the telomeres of specific chromosomes?

Is it possible to sequence the telomere of a specific chromosome with precision say, using a primer starting from the subtelomeric region?

05 June 2018 1,849 0 View

Gardella gel electrophoresis - can we load total genomic DNA instead of lyse cells in-well?

Will the gel be able to separate out chromosomal/episomal/linear DNA if we load total genomic DNA (~5 micrograms) instead of lysing the cells within the wells?

02 March 2018 504 3 View

How does viral load change with each cell line passage?

06 July 2015 2,945 3 View

How do I directly perform sequencing PCR on a PCR product without going through cloning?

Need some help here. I am trying to verify if the band on my PCR gel is correct or not. What is the fastest/easiest way to do it? Please advise.

07 August 2014 6,602 11 View

I need a step by step guide to PCR primer design

My project involves screening for novel SNPs in tumor samples. I've identified a novel SNP locus in a particular gene. How can I proceed to design primers for PCR testing on a bigger tumor sample?...

10 November 2013 3,523 4 View

Ways to generate drug-resistant cancer cell lines?

I need advice here. Anyone has any good/detailed protocols on developing drug-resistant cancer cell lines? I am interested in creating such cell lines using sub-toxic drug doses at incremental...

07 August 2013 6,209 40 View

How to promote research work and scholarly activity in a busy clinical residency program?

How to promote research work and scholarly activity in a busy clinical residency program? Most people are just too busy or are not keen in the first place. Any suggestions?

05 June 2013 8,715 25 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View