Gardella gel electrophoresis - can we load total genomic DNA instead of lyse cells in-well?

Will the gel be able to separate out chromosomal/episomal/linear DNA if we load total genomic DNA (~5 micrograms) instead of lysing the cells within the wells?

02 March 2018 504 3 View

Where to obtain antibodies to Human Herpesvirus-7 (HHV-7)?

Hi all. I'm looking for antibodies to HHV-7 for immunohistochemistry and other protein work. If anyone has any other resources it will be greatly appreciated too. Best, - Jason

07 August 2016 9,986 2 View

How does viral load change with each cell line passage?

06 July 2015 2,945 3 View

How do I directly perform sequencing PCR on a PCR product without going through cloning?

Need some help here. I am trying to verify if the band on my PCR gel is correct or not. What is the fastest/easiest way to do it? Please advise.

07 August 2014 6,602 11 View

I need a step by step guide to PCR primer design

My project involves screening for novel SNPs in tumor samples. I've identified a novel SNP locus in a particular gene. How can I proceed to design primers for PCR testing on a bigger tumor sample?...

10 November 2013 3,523 4 View

Ways to generate drug-resistant cancer cell lines?

I need advice here. Anyone has any good/detailed protocols on developing drug-resistant cancer cell lines? I am interested in creating such cell lines using sub-toxic drug doses at incremental...

07 August 2013 6,209 40 View

How to promote research work and scholarly activity in a busy clinical residency program?

How to promote research work and scholarly activity in a busy clinical residency program? Most people are just too busy or are not keen in the first place. Any suggestions?

05 June 2013 8,715 25 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View