Is it possible to sequence the telomere of a specific chromosome with precision say, using a primer starting from the subtelomeric region?
Will the gel be able to separate out chromosomal/episomal/linear DNA if we load total genomic DNA (~5 micrograms) instead of lysing the cells within the wells?
02 March 2018 553 3 View
Hi all. I'm looking for antibodies to HHV-7 for immunohistochemistry and other protein work. If anyone has any other resources it will be greatly appreciated too. Best, - Jason
07 August 2016 10,030 2 View
Hi all. I am performing an experiment to detect the presence of viral DNA in a cell line model. I am wondering when will be the best time to harvest the cells so as to achieve the highest viral...
06 July 2015 2,998 3 View
Need some help here. I am trying to verify if the band on my PCR gel is correct or not. What is the fastest/easiest way to do it? Please advise.
07 August 2014 6,653 11 View
My project involves screening for novel SNPs in tumor samples. I've identified a novel SNP locus in a particular gene. How can I proceed to design primers for PCR testing on a bigger tumor sample?...
10 November 2013 3,573 4 View
I need advice here. Anyone has any good/detailed protocols on developing drug-resistant cancer cell lines? I am interested in creating such cell lines using sub-toxic drug doses at incremental...
07 August 2013 6,263 40 View
How to promote research work and scholarly activity in a busy clinical residency program? Most people are just too busy or are not keen in the first place. Any suggestions?
05 June 2013 8,757 25 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View