I am trying to build an E. coli strain where I control expression of three different products from three inducible promoters present on three compatible plasmids. I already have Plasmid 1: Plac, colE1 ori, Amp; Plasmid 2: Ptet, p15A ori, Cm. I am looking for another plasmid which has to contain a compatible ori such as IncFII or pT181, and preferably have a pBAD promoter and Tet, Spec or other distinct selection markers. If anybody knows of some commercial vectors that would be great. If you know of people who can kindly share something like this, would be even better. Thanks in advance for any help.
Novagen pRSFDuet vector carrying RSF origin with kan resistance fits your requirement.
Max, you could also use pBR322 or pUC, John's lab have a wide variety of them.
John had made a variety of pBAD322 vectors (with rol fixed so less copy numbers, and a good variety of different antibiotic resistance).
Thanks Yaoqin, John emailed me two days ago with the paper on pBAD322, its very helpful, i might ask you (or someone in your lab) for one of those vectors, just need to decide on the selective marker I would want to use.
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