Maksym Bobrovskyy

4 Questions 20 Answers 0 Followers

Questions related from Maksym Bobrovskyy

Where to find a third compatible plasmid that would work with colE1 and p15A ori?

I am trying to build an E. coli strain where I control expression of three different products from three inducible promoters present on three compatible plasmids. I already have Plasmid 1: Plac,...

05 May 2016 8,221 5 View

Can E. coli grow on Aspartate as a sole carbon source?

Would supplementation with Asp improve growth of E. coli on minimal glycerol media? Can Bacillus subtilis grow on Asp as sole carbon source? Paper suggestions would be great too. Thanks!

07 July 2013 3,684 1 View

Does lead acetate (PbAc) degrade after it has been dissolved in water?

I use PbAc for RNA digestion. I prepare a fresh solution of PbAc bore every experiment because that is what it says in the protocol. I wonder if that's really necessary or simply a precaution. It...

10 October 2012 3,977 1 View

Why do my pictures of large sequencing polyacrylamide gels come out blurry?

I am detecting digested, P32 terminally labelled RNA using large polyacrylamide gel. I prerun the gel for 1 hr to make sure it's hot and run my gel for ~2 hrs in 1x TBE buffer at 1600V. I expose...

10 October 2012 5,787 4 View