What is the flash point of chloroform?

More Sandip Salunkhe's questions See All

CAN WE MIX Single stained beads for FLUROSCENCE MINUS ONE CONTROL (FMO) in flow cytometry? ?

Hi, For compensation, we can use single-stained beads in flow cytometry. For gating control or FMO: can we just mix single-stained beads to get FMO?

25 May 2024 6,832 1 View

Why the solubility of chitosan is poor and how it affects the grafting process?

why the solubility of chitosan is poor, and how does it affect the grafting process?

22 April 2024 1,207 1 View

Where to clone guid RNA sequence in plasmid GRNA cassete?

In 84232 add gene plasmid, I am not sure at which site should I clone guide RNA

04 April 2024 8,044 3 View

Where to clone guid RNA sequence in plasmid GRNA cassete?

In addgene 84323, plasmid they have empty gRNA casette. I am not sure where to clin guide RNA of interest. 1. Is it should be between U6 promoter and gRNA casette or 2. In gRNA casette itself?

04 April 2024 8,157 3 View

What is the possibility of status for vegetation in the abandoned mines? If it the research gap for Nepal, what can be included in results?

Marble mining have been abandoned since 2015 in Lalitpur Municipality of Nepal. Under my thesis, I have decided to check the status of vegetation in the Mine. But, I am not quite confident what...

18 March 2024 1,968 0 View

How can revenue of Indian railways be increased?

Explanation: Only 57% of the expenses for urban trains and 40% for suburban trains have been recuperated, a fact that regular train travelers might have observed through ticket prices. Despite...

24 November 2023 5,995 2 View

Modeliing PM10 and PM 2.5?

How best a judgement can be made based on modelling PM10 and PM2.5

15 October 2023 6,552 0 View

Does the ibrav change when we use vacuum for an atomic system in a quantum espresso input file using nanolabo?

Normally for ZnO, ibrav = 4 (i.e hexagonal). Now I want to perform gas sensing studies. So I create a vacuum of 10A in nanolabo where the atoms are placed. The ibrav now changes to 12 (i.e...

12 September 2023 3,180 0 View

Can we speak to animals?

Animals are enigmatic beings to us. Their emotions remain a mystery, and their communication methods are beyond our comprehension. However, consider the possibility of an individual who has an...

09 September 2023 4,943 6 View

How to apply WPM for data set which contains 0 ?

I am trying to Multi-criteria Analysis for seismic vulnerability assessment where retrofitting done or not is one of the criteria. Here I have assigned 0 for no and 1 for yes response. Similarly...

03 July 2023 305 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

Joel Berzas

See link below.

https://www.google.com/#q=flashpoint+of+chloroform

Jack Silver

It has no flash point- not flammable by itself.

See: https://pubchem.ncbi.nlm.nih.gov/compound/chloroform#section=Melting-Point

Nizar Matar

Chloroform dos not have a flash point. Saying this does not mean a relax on precautions since CHCl3 bottle usually has some ethanol "as stabilizer" and ethanol is volatile & flammable.

As you know, chloroform was used as general anesthetic & then skipped. So my advice is to try another solvent for the drug such as dichloromethane "DCM" CH2Cl2. Of course, DCM will be disposed after it does its job.