Why are my Maxent SDM projections coming up almost entirely blue?

Hello Maxent Community, I have been generating SDM models and projections in Maxent. I have had some success with one set of variables (some Hydroshed variables mixed with World CLIM variables);...

19 January 2021 3,994 7 View

Expression differences between Western Blot and proteomics?

I recently did a proteomics experiment on lung cancer cells examining the effect of knocking down my protein of interest, and did a Western blot and qPCR to confirm the knockdown prior to prepping...

14 December 2019 547 3 View

What is the best equipment to use for tracking jaguars with radio telemetry and GPS?

I currently work as a wildlife biologist in the Peruvian Amazon, and I have recently begun to explore the possibility of capturing a jaguar and fitting it with a VHF/GPS collar in order to study...

30 April 2019 3,086 2 View

What can I use as a test sample for PALM/STORM super-resolution?

13 March 2017 843 4 View

Has anyone performed Direct PCR with bird blood stored in ethanol?

06 April 2016 2,922 1 View

How can I optimize PCR for 16S rDNA on low template concentrations?

I'm trying to amplify 16S rDNA from total DNA extracted from Ixodes ticks. Due to an error in the extraction process, some of my DNA concentrations are very low, in the range of 5 to 0.5 ng/uL....

16 February 2016 9,098 3 View

Where can I find an explanation for the dangers of averaging ranks from different measures?

I have several coworkers who are not statisticians that want to take ranks of measure from a variety of sources and measuring different concepts and average them together to provide an overall...

14 June 2015 6,550 4 View

Can anyone help me with information regarding fire protection methods in listed buildings?

I am looking for literature in the form of peer-reviewed articles, scientific institution publications, government publications, etc. regarding fire protection solutions for listed buildings. if...

26 February 2014 4,286 4 View

The microbiome amd human diseases

Viruses, bacteria and other pathogens are implicated in a number of human diseases including Alzheimer's disease, epilepsy, muliple sclerosis and Parkinson's disease, ADHD, autism, bipolar...

14 May 2011 2,173 4 View

KEGG pathway analysis of Bipolar disorder and multiple sclerosis susceptibility genes

These pathways, etched out by several hundred genes, relate to brain pathologies and transmitter networks , but also to the immune system and pathogen defense and entry...

11 March 2011 2,106 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View