The robust standard errors were calculated using MLR.
Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...
07 July 2024 9,206 4 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
Could you share other peer-reviewed journals that have accepted your scholarship? I am seeking to publish a new article focused on diversity and student engagement or write a book chapter. Any...
19 June 2024 4,716 0 View
Systematic review
21 March 2024 5,901 1 View
I am interested in looking at male attachment, including the development of attachment wounds, systemic and cultural factors. I’d like to specifically tie together the potential impact of healing...
10 February 2024 656 2 View
Hello everybody, I'm having trouble cloning a 700bp insert into a 18kb plasmid. I'm using Neb Hifi assembly (Gibson assembly) but everytime I have no colonies on the plate. I tried many different...
08 August 2023 5,490 7 View
Hello, I have extracted RNA from the Intestinal Enteroid Cell Line and have measured the expression of multiple genes using RT-qPCR. I tested two housekeeping genes GAPDH and ACTBR both showed...
07 December 2022 5,910 6 View
We are finding difficulties in neutrophils activating and clumping once resuspended either from a fresh sample or a frozen/thawed sample. Sample is acquired from human blood using Sepmate tube and...
18 October 2022 2,820 1 View
Hello, I'm currently trying to delete a 5kb gene from my plasmid, using the Directed-site mutagenesis kit from neb but the PCR part is not working and I don't know why. I designed the primers...
07 March 2022 6,130 4 View
Hello Maxent Community, I have been generating SDM models and projections in Maxent. I have had some success with one set of variables (some Hydroshed variables mixed with World CLIM variables);...
19 January 2021 4,397 7 View
For people today, the enigma of the size of the proton still remains enigmatic. However, being myself a researcher in new physical theory which makes it possible to extend the different standard...
28 July 2024 7,823 1 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
Dear all, I have a user written estimation code based on the paper dynamic panel data estimations with fixed effect by Galvao(2009). I have seen many papers using this method and pvalue, standard...
22 July 2024 4,588 1 View
I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.
21 July 2024 3,465 5 View
Dear Community, I would like to develop and validate a qualitative NMR method for the analysis of a specific category of chemicals, and my question is the following: What are the criteria that I...
08 July 2024 1,402 4 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...
01 July 2024 3,267 1 View
Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...
30 June 2024 1,569 1 View
Good morning/day/evening salaam I’d like to ask about the FRAP assay again. I have read some articles which represent the data of frap assay as mg trolox equivalent/L, mg ascorbic acid...
27 June 2024 5,239 0 View
I am getting a positive correlation between two variables. But in Multi-linear regression, i find the regression coefficient to be negative between those two variables. Is this normal?
25 June 2024 9,250 4 View
