Where I can order fungal ITS1 and ITS4 primers?

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Sibnarayan Datta

Hi,

You can get the primers synthesized by any reputed genomics service provider that have primer synthesizing service in your city/ country. You just have to email the primer sequence to them. Try to get at least PAGE purified primers or if you have sufficient funds, then go for HPLC purified primers.

Best of luck.

Andrew Taylor

I always use Sigma (https://www.sigmaaldrich.com/configurator/servlet/DesignCenter?customName=customoligo) - they are cheap and usually fast. If they are just for PCR then desalt is fine. We routinely use ITS1F and ITS4 for fungi.