Where I can order fungal ITS1 and ITS4 primers?

More Olena Myronycheva's questions See All

SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

Hi, help please, how to cancel the authorship of the article Civilizational Phenomenon of Narcissism: Vectors of Objectification in a Paradigm...?

Hi, help please, how to cancel the authorship of the article Civilizational Phenomenon of Narcissism: Vectors of Objectification in a Paradigm of Psychoanalysis ?

15 December 2019 9,043 3 View

Could the gene silencing be avoided when it is expressed from the episomal plasmid?

In the case of yeast transformants

14 March 2016 9,434 1 View

Does anyone know some kits for express diagnostics basic soil characteristics?

Dear colleagues, I need to estimate the basic soil characteristics - total carbon, nitrogen, potassium content. I also need the content of Fe and halogens (F, Cl, maybe I). The point is that I can...

12 February 2015 4,874 9 View

Has anyone used Mesquite for combining molecular and morphological data? Do you know a algorithm for doing that?

Phylogenetic analysis (combining molecular and morphological data).

10 August 2014 4,406 7 View

Could you please suggest a technique of a plant root preparation for XRF confocal imaging?

Zou Finrock and Kelly Summers have recommended me to contact you. We are using 8-day old Arabidopsis plants to see of the effects of toxic metals, in the root tips, in particular. They are really...

01 January 1970 5,600 3 View

What do you think about the status of ownership in the charing economy?

We understand that in sharing economy, access to usage will be important. How will the attitude to ownership and disposal of property change? Do you want be the owners?

01 January 1970 546 8 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Sibnarayan Datta

Hi,

You can get the primers synthesized by any reputed genomics service provider that have primer synthesizing service in your city/ country. You just have to email the primer sequence to them. Try to get at least PAGE purified primers or if you have sufficient funds, then go for HPLC purified primers.

Best of luck.

Andrew Taylor

I always use Sigma (https://www.sigmaaldrich.com/configurator/servlet/DesignCenter?customName=customoligo) - they are cheap and usually fast. If they are just for PCR then desalt is fine. We routinely use ITS1F and ITS4 for fungi.

Olena Myronycheva

Thank you very much for the pathway.