Payel Das

6 Questions 3 Answers 0 Followers

Questions related from Payel Das

Immunoprecipitation and mass spec in Knockdown cells?

I have generated stable knockdown MCF7 cells of a protein using lentivirus and observed slower growth and inhibition of Raf-MAPK pathway. I want to understand if I can do IP from WT MCF7 and KD...

06 August 2025 5,611 0 View

Is it possible to study glucose intake and insulin sensitivity in MCF-7 cells?

I found a HDAC in mitochondria of MCF-7 and was thinking of directing my research hypothesis towards insulin sensitivity and glucose uptake. Is it good model to study insulin sensitivity?

12 April 2025 5,685 0 View

Can we mount transient transfected cancer cells expressing GFP fusion protein with prolong glass antifade without fixation & permeable nucleus dye?

To avoid damage to cells and loss of GFP fluorescence intensity or damage of organelles I wanted to avoid fixation with ethanol or 4% formaldehyde. Is it okay to stain cancer cells live with live...

20 March 2025 5,756 0 View

What can be a loading control for subcellular fractions?

I am doing subcellular fractionation of nucleus, cytosol, mitochondria. To verify equal loading of lysates what loading control can be used?

19 March 2025 3,297 3 View

How to find out transcript isoforms of a gene? Microarray can be used?

I want to find out different isoforms of a gene. I know microarray or RNA seq can be used. But I need clarification or any other methods that can be used.

04 March 2025 1,443 2 View

Where can I obtain Leishmania braziliensis or Trypanosoma brucei or Trypanosoma cruzi strain?

I need any one of these strains for my research.

22 May 2024 8,030 0 View