I am looking for software that can be used to calculate leaf area for a plant without damaging the plant. The "destructive method and the graph sheet method" are tedious and time consuming. Does anyone have any experience with this?
You can take a photo of the leaf against a background with good contrast (a sheet of paper) with a ruler for scale in the image, and then you can calculate leaf area using ImageJ, as Briony mentioned.
This video shows one relatively quick way of doing it (there are others)
If you are always studying the same species, you can do a calibration curve by measuring actual area, length and width of a leaf, and then you'll only have to measure length and with and enter them in the regression equation
ImageJ (free) is highly recommended. very customisable and If you take all the pictures at the same focal distance you can batch process the whole lot quite quickly.