What is a good RNA extraction method for RNA-Seq (especially QuantSeq)?

Which RNA extraction method would you recommend for RNA-Seq of snap-frozen fetal murine tissues? We will be doing Lexogen QuantSeq. Considering Qiagen RNeasy (good, but pretty expensive) or Thermo...

09 October 2019 779 3 View

What is the most powerful fluorescent detection system for IF and FISH?

I'm looking for solutions to maximise the signal-to-noise ratio in immunofluorescence and/or FISH. We are routinely using Alexa Fluor labelled secondary antibodies but now we need something more...

10 November 2018 7,062 6 View

What is the most effective detection system for 16S FISH?

We are setting up 16S FISH for animal tissues with low bacterial abundances. What is the most effective & specific system for probe detection & signal amplification?

03 April 2018 6,214 1 View

What is the best virtual microscopy solution for teaching histology & pathology?

We are setting up a virtual microscopy platform for teaching histology & pathology in a university setting. We need to serve ~50-100 simultaneous online users with a collection of ~200 slides...

01 February 2018 7,200 15 View

Are there any broad-spectrum anti-bacterium antibodies?

Are there any antibodies that can be used to detect broad groups of bacteria in tissue sections or flow cytometry? Like, ANY bacterium (as opposed to animal cells), or gram+ / gram- bacteria, or a...

08 September 2017 6,783 2 View

Can a very strong promoter cause LOWER total luminescence in dual luciferase assay?

We are testing various promoters in a dual luciferase assay. Surprisingly, a promoter expected to be strong shows very low luminescence for both the test construct AND the control (the levels of...

02 March 2017 2,305 0 View

How to extract bacterial DNA from swabs using bead beating?

We are happily extracting bacterial DNA using a bead beating protocol. Now we need to start processing swab samples containing fecal etc bacteria. If we put the entire swab into the bead beating,...

05 June 2016 8,551 5 View

Any advice on showing bacteria in intestinal paraffin sections?

I'm trying to visualize intestinal bacteria in paraffin sections. The problem is, the bacterial cells seem to shrink so much that no shapes are preserved, they are all just tiny roughly circular...

03 April 2015 1,541 12 View

What would be the simplest possible protocol to lyse cells for genomic PCR, with minimal pipetting steps?

I'm trying to devise the most streamlined protocol possible for PCR screening of mammalian cells grown on 96-well plates. My current lysis protocol is as follows: Dilute the cell culture medium...

10 November 2014 3,516 21 View

How to visualize bacteria in histological animal sections (cryo or paraffin)?

Hello! What is the best histological stain or immunostaining marker to visualize bacteria in sections of animal tissues? Optimally, staining all (or most) bacteria but no eukaryotic cells, and...

09 October 2014 8,179 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View