21 Questions 34 Answers 0 Followers
Questions related from Mikael Niku
Which RNA extraction method would you recommend for RNA-Seq of snap-frozen fetal murine tissues? We will be doing Lexogen QuantSeq. Considering Qiagen RNeasy (good, but pretty expensive) or Thermo...
10 October 2019 861 3 View
Does anyone know a resource for data on organ sizes (mass, preferably also dimensions) for fetal mice at various stages of development?
03 March 2019 1,601 1 View
I'm looking for solutions to maximise the signal-to-noise ratio in immunofluorescence and/or FISH. We are routinely using Alexa Fluor labelled secondary antibodies but now we need something more...
11 November 2018 7,124 6 View
We are setting up 16S FISH for animal tissues with low bacterial abundances. What is the most effective & specific system for probe detection & signal amplification?
04 April 2018 6,280 1 View
We are setting up a virtual microscopy platform for teaching histology & pathology in a university setting. We need to serve ~50-100 simultaneous online users with a collection of ~200 slides...
02 February 2018 7,294 15 View
Are there any antibodies that can be used to detect broad groups of bacteria in tissue sections or flow cytometry? Like, ANY bacterium (as opposed to animal cells), or gram+ / gram- bacteria, or a...
09 September 2017 6,855 2 View
We are testing various promoters in a dual luciferase assay. Surprisingly, a promoter expected to be strong shows very low luminescence for both the test construct AND the control (the levels of...
03 March 2017 2,397 0 View
We are happily extracting bacterial DNA using a bead beating protocol. Now we need to start processing swab samples containing fecal etc bacteria. If we put the entire swab into the bead beating,...
06 June 2016 8,620 5 View
I'm trying to visualize intestinal bacteria in paraffin sections. The problem is, the bacterial cells seem to shrink so much that no shapes are preserved, they are all just tiny roughly circular...
04 April 2015 1,619 12 View
I'm trying to devise the most streamlined protocol possible for PCR screening of mammalian cells grown on 96-well plates. My current lysis protocol is as follows: Dilute the cell culture medium...
11 November 2014 3,569 21 View
Hello! What is the best histological stain or immunostaining marker to visualize bacteria in sections of animal tissues? Optimally, staining all (or most) bacteria but no eukaryotic cells, and...
10 October 2014 8,246 6 View
We are planning to start using slide scanner images for histology teaching (high-res scans of entire tissue sections). Can you recommend applications for storage and online sharing of this kind of...
02 February 2014 9,344 1 View
I would be especially interested in the color quality of the images. I'm trying to find a new camera for our histology teaching laboratory, and in this case we need live full HD image with good...
12 December 2013 2,611 2 View
I'm in the process of cloning suspension cells and I need to use PCR for screening successful clones. What would be the easiest way to collect samples from 96-well plates, let's say about 5 to 10...
11 November 2013 8,224 3 View
I'm having trouble with contamination of linearized plasmid with the intact circular plasmid. I have heavily overdigested the plasmid, gel purified, and digested with another restriction enzyme,...
11 November 2013 893 6 View
I'm looking for a camera for our histology teaching lab (for showing the slides on the screen). Need a fast live image with good colour reproduction and adequate resolution, plus a simple and easy...
09 September 2013 2,857 17 View
I'm using sodium butyrate in cell culture, but I'm slightly worried about the stability of my reagents. There is very conflicting information available regarding its stability in aqueous or...
03 March 2013 8,991 2 View
We have been analyzing an immunoglobulin locus of a veterinary species, where the exact locus structure is not available. We know that in four individual animals we have X different sequences...
02 February 2013 7,949 0 View
antibody, detected using the Li-cor Odyssey infrared system. We have used the instrument previously only
02 February 2013 2,308 14 View
Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with...
02 February 2013 7,852 16 View
I have problems getting very weak fluorescence signal from my probe-based RT-qPCR assay. The Ct is not bad (less than 30, and I see a clear although weak band on gel), but the signal intensity is...
01 January 2013 3,839 4 View
